Tg(IghelMD4)4Ccg
Transgene Detail

Summary

• Symbol: Tg(IghelMD4)4Ccg
• Name: transgene insertion 4, Christopher C Goodnow
• MGI ID: MGI:2384162
• Synonyms: Hel-Ig, HELmuMT, Hy10, IgHEL, Ig^HEL, Ig^Tg, Ig-transgenic, MD-3, MD4, MD4 HEL Ig, MD4 Ig, MD4 Ig BCR Tg, MD4-RAG, SWHEL
• Transgene: Tg(IghelMD4)4Ccg Location: unknown Genetic Position: Chr17, Syntenic
• Alliance: Tg(IghelMD4)4Ccg page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The hybridoma HyHEL10 produces an antibody with high affinity for hen egg lysozyme. Genomic clones encoding HyHEL10 immunoglobulin heavy and light chains were used for this construct. The gamma1-constant region of the heavy chain was replaced by a segment containing the mu and delta constant region genes derived from BALB/c mice. The light and heavy chains were co-injected into C57BL/6 eggs. Five lines, MD3-MD7, that carried multiple copies of the transgene construct were created. The MD4 line had the highest expression of the transgene and was propogated for further studies. Mice carrying this transgene had high serum titers of anti-lysozyme antibodies. (J:78308)
• Inheritance: Not Specified

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available

References

• Original: J:78308 Goodnow CC, et al., Altered immunoglobulin expression and functional silencing of self-reactive B lymphocytes in transgenic mice. Nature. 1988 Aug 25;334(6184):676-82
• All: 289 reference(s)