Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck
Transgene Detail

Summary

• Symbol: Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck
• Name: transgene insertion 2, Donald A Cohen
• MGI ID: MGI:3051865
• Synonyms: Mafia, MaFIA (Macrophage Fas-Induced Apoptosis), Tg(Csf1r-GFP, NGFR/FKBP12)2Bck
• Transgene: Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck Location: unknown
• Alliance: Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck expresses 3 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  FAS (355)

  Fas	(MGI:95484)

  human

  FKBP1A (2280)

  Fkbp1a

  (MGI:95541)

  human

  NGFR (4804)

  Ngfr	(MGI:97323)

• Mutation details: The transgene's bicistronic transcript, expressed from the promoter of the macrophage-expressed colony stimulating factor 1 receptor gene, encodes enhanced green fluorescent protein (EGFP) and a conditionally apoptotic fusion protein. The latter comprises the extracellular and transmembrane domains of the human low affinity nerve growth factor receptor, two tandem copies of the human FK506 binding protein, and the intracellular domain of human TNFRSF6 - the Fas receptor protein - and is translated from an internal RNA expression signal (IRES) inserted at the translation start codon of the nerve growth factor receptor (NGFR) coding sequence. (J:89035)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Homozygous mutant mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities.

The transgene is expressed constitutively in macrophages and dendritic cells and permits conditional ablation of these cell populations in the mouse. Oligomerization of the fusion protein by binding of a bivalent FK506 analog causes them to undergo apoptosis via the Fas-mediated pathway.

In transgenic mice treated with the dimerizing reagent AP20187, EGFP fluorescing peritoneal and bone marrow macrophage numbers are depleted by more than 90%, and macrophage numbers in blood, spleen, lung and thymus by more than 70%. Seven days after cessation of treatment, the EGFP fluorescing macrophage and dendritic cell populations rebound.

References

• Original: J:89035 Burnett SH, et al., Conditional macrophage ablation in transgenic mice expressing a Fas-based suicide gene. J Leukoc Biol. 2004 Apr;75(4):612-23
• All: 57 reference(s)