Experiment
Genetic loci associated with long bone length, necropsy body weight, and organ weight were mapped in 1045 animals from a (LG/J x SM/J)F2 intercross. Animals were phenotyped between 70 and 237 days of age, with an average age at sacrifice of 144 days. Twodifferent sets of F2 mice were genotyped for 75 and 96 polymorphic markers across 19 autosomes. 97 single trait QTL were identified. After pleiotropy analysis the 97 loci coalesced into 35 pleiotropic QTLs. Cutoff for statistical significance is LPR=3.49but all pleiotropic loci listed in the reference are reported here.
On mouse Chromosome 19, linkage to spleen weight, ulna length (female-specific), and femur length (female-specific) mapped to 15 cM near D19Mit16 (LPR=2.66). This locus is named Bod11 (bone length and organs 11). The Bod11 confidence interval spans 0 cM - 66 cM. Potential candidate genes in this region include Foxb2 (10.5 cM), Rfk, Tmc1 (15 cM), and Fgf8 (45 cM).
Analysis Tools