Experiment
Genetic loci associated with long bone length, necropsy body weight, and organ weight were mapped in 1045 animals from a (LG/J x SM/J)F2 intercross. Animals were phenotyped between 70 and 237 days of age, with an average age at sacrifice of 144 days. Twodifferent sets of F2 mice were genotyped for 75 and 96 polymorphic markers across 19 autosomes. 97 single trait QTL were identified. After pleiotropy analysis the 97 loci coalesced into 35 pleiotropic QTLs. Cutoff for statistical significance is LPR=3.49but all pleiotropic loci in the reference are reported here.
On mouse Chromosome 2, linkage to organ weight and bone length, Bod1 (bone length and organs 1), mapped to 40.4 cM near D2Mit380 (LPR=5.62). This locus affects liver and kidney weight (male-specific), body weight at necropsy, and femur and ulna length. The confidence interval of Bod1 spans 62 cM - 86 cM.
Linkage to tibia and humerus lengths, Lbn2 (long bones 2) mapped to 78.2 cM near D2Mit28 (LPR=8.7). The effect of Lbn2 on humerus length is male-specific. The confidence interval spans 92 cM - 114 cM. Previously identified QTL Skl2 at 69 cM may be identical to Lbn2. Potential candidate genes for Lbn2 include Bmp2 (76.1 cM), Pax1 (82 cM), and Fgf7.
Linkage to spleen weight in males mapped to 84 cM near D2Mit22 (LPR=2.84). This locus is named Org1 (organ weight 1). The Org1 confidence interval spans 108 cM - 144 cM.
Analysis Tools