Experiment
Genome scan was performed on 218 (C57BL/6J x DBA/2J)F2 intercross animals using 101 microsatellite markers to identify QTLs associated with alcohol preference. Parental strain C57BL/6J exhibits higher alcohol preference compared to parental strain DBA/2J. Significant alcohol preference QTLs mapped to mouse Chromosome 1 spanning 45 cM - 95 cM (Ap1q, LOD = 4.5 between D1Mit194 and D1Mit110), mouse Chromosome 4 spanning 65 cM - telomere (Ap3q, LOD = 4.7 at D4Mit42), and mouse Chromosome 9 spanning 10 cM - 35 cM (Apq5, LOD = 4.8 cM at D9Mit330). Suggestive QTLs mapped to mouse Chromosome 2 spanning 30 cM - 50 cM (Ap2q, LOD = 3.1 at D2Mit61), mouse Chromosome 10 (Ap4q) spanning 18 cM - 57 cM, and mouse Chromosome 3 spanning 38 cM - 60 cM (Ap6q, LOD = 3.4 between D3Mit12 and D3Mit86). Ap1q, Ap2q, Ap3q, and Ap4q were provisionally mapped in BXD RI strains and confirmed in the present study, while Ap5q and Ap6q are novel. C57BL/6J-derived alleles increases alcohol preference at all loci mapped. Ap1q is follows adominant mode of inheritance and maps near Chrnd (Acrd), Chrng (Acrg), and Htr5b. Ap2q is consistent with a recessive or additive mode of inheritance and maps near Scn1a, Scn2a, Scn3a, and Scn7a . Ap3q is consistent with a dominant or additive mode of inheritance and maps near Htr1da, Htr1db, Ahd1 (Ssdh1), and Tas1r3 (Sac). Ap5q is consistent with a dominant or additive mode of inheritance and maps near Htr1b and Drd2. Ap6q is most consistent with an additive mode of inheritance and maps near the alcohol dehydrogenase gene complex at approximately 71.2 cM on mouse Chromosome 3.
Analysis Tools