Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  19
• Reference
  J:68175 Rieger DK, et al., A double-deletion mutation in the pitx3 gene causes arrested lens development in aphakia mice. Genomics. 2001 Feb 15;72(1):61-72
• ID
  MGI:1934243

Genes

Gene	Assay Type
Gbf1	direct sequencing
Pitx3	direct sequencing
Elovl3	direct sequencing

Notes

• Experiment
  The authors were able to establish tight linkage between pitx3 and the ak mutation. Expression analysis revealed a decrease in the Pitx3 transcript in ak mice. Subsequent sequence analysis revealed the presence of two deletions in the Pitx3 gene in ak mice that remove the putative promoter region, exon 1 and intron 1 and a region 2.5kb upstream of the putative transcription site. The order of genes around the Pitx3 locus was determined to be: 5' - Gbf1 - 3' - 2.5kb - 5' - Pitx3 - 3' - 3' - Elovl3 - 5'.