Experiment
Authors screened DNAs from 73 T-cell lymphomas induced by gamma-rays in (C57BL/6J x BALB/cJ)F1 mice and the reciprocal (BALB/cJ x C57BL/6J) mice for loss of heterozygosity (LOH) with several polymorphic markers. Significant differences in LOH was observed with Chromosome 4 markers compared to markers on other chromosomes. 12 different patterns of LOH were seen with allele losses on Chromosome 4. Analysis of the LOH data revealed three different non-contiguous areas of allele loss. These were called Tlsr1(Chi square = 87.77, d.f. = 1, P<0.0000001), Tlsr4 (Chi square 106.16, d.f. = 1, P<0.0000001) and Tlsr5 (Chi square = 52.77, d.f. = 1, P<0.0000001). Analysis of LOH around the Tlsr1 region gave a probable order of markers in this region as: Centromere - D4Mit77 , D4Mit245 - Cdkn2b - (exon beta) - Cdkn2a (exon alpha) - D4Wsm1.
10.20.2015 Curator Note: Because Tlsr1 was originally mapped in J:31797 in 1996 using (C57BL/6J x RF/J)F1 mice, which differ from the population used here, we consider the current study a separate mapping experiment and have named this QTL Tlsr9.
The region centered at D4Mit116 was called Tlsr4 while the region encompassing D4Mit21 was called Tlsr5. To reinforce the signifance of allele losses at D4Mit21, additional allelotype analysis on 49 gama-radiation induce T-lymphomas of the (C57BL/6J x RF/J)F1 was performed. A higher Chi square value of 79.27 and p<0.0000001 was obtained. Tgfbr1 is a putative candidate for a tumor suppressor gene in the Tlsr5 region.
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