Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  2
• Reference
  J:47082 Latham KE, et al., Localization of genes encoding egg modifiers of paternal genome function to mouse chromosomes one and two. Development. 1998 Mar;125(5):929-35
• ID
  MGI:1328189

Genes

Gene	Assay Type
Egm2	visible phenotype
D2Mit14a	reported elsewhere
Mdk	reported elsewhere
D2Nds1	reported elsewhere
D2Mit12	RAPD

Notes

• Experiment
  Female animals from 25 BXD (B=C57BL/6J;D=DBA/2J) recombinant inbred (RI) strains were mated with (C57BL/6J x DBA/2J)F1 males and the fertilized eggs were subjected to nuclear transplantation to create androgenetic and gynogenetic embryos. The frequency of androgenetic blastocyst formation was measured. Eggs from parental strain C57BL/6J supports a significantly higher rate of androgenetic blastocyst formation compared to eggs from DBA/2J (50% vs 6%).
  
  The analysis of the SDPs in the BXD RI lines was informative to show the Egm2 (egg modifier locus 2) phenotype was associated with mouse Chromosome 2 markers D2Mit14a (48.5 cM), Mdk (53 cM), D2Nds1 (53 cM) and D2Mit12 (53 cM). Therefore, the QTL interval of Egm2 is limited to 48.5 cM - 53 cM. The following strains were DBA/2J-like - 5, 12, 19, 20, 21, and 29, the rest with the exception of strain 13 were C57BL/6J-like. The strain distribution supports a two locus model with another region associated with Chromosome 1 named Egm1 (egg modifier locus 1).