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FACS 2 for Project: NIH-1304
Test Description: The mononuclear cell profile is derived by staining a single sample of lysed peripheral blood from each mouse with a panel of six lineage-specific antibodies: CD45 PerCP, anti-TCRb APC, CD4 PE, CD8 FITC, pan-NK PE, and CD19 FITC. The two FITC and PE labeled antibodies stain mutually exclusive cell types. The samples are analyzed using a Becton Dickinson FACSCalibur flow cytometer with CellQuest software.


Results

TCRbeta+ T-Cells




TCRbeta- Cells




  FACS Analysis data for Project: NIH-1304 - Results        Export Data to Spreadsheet
Mouse
Label 		Genotype Age (days) CD4+T CD8+T CD4+CD8+T CD4-CD8-T NK cells B cells Monocytes Image Thumbnail
M-85 het 97 17.51 9.34 0.87 0.92 5.31 61.52 4.53 None None
F-88 het 97 21.19 14.9 1.59 0.25 3.78 56.17 2.13 None None
F-77 hom 97 35.09 20.7 3.06 0.66 5.36 30.27 4.87 None None
M-82 hom 97 29.58 11.83 1.14 0.67 6.19 46.46 4.13 None None
M-89 wt 97 31.62 13.15 1.74 0.23 5.36 43.54 4.36 None None
F-101 wt 97 27.9 20.43 0.98 0.4 4.14 41.54 4.61 None None
 


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Lexicon Phenotypic Analysis software version 05.05.00.05