Test Description: Bacterial lipopolysaccharide (LPS) is an endotoxin, a potent
inducer of the acute phase response and systemic inflammation. The mice will:
- Be injected intraperitoneally (i.p.) with a sublethal dose of LPS in
200 µL sterile saline using a 26 gauge needle.
- The dose will be based on the average weight of the mice tested at 1 µg/g body weight
3 hours after injection, a 100ul blood sample will be taken and analyzed for the
presence of TNFα, MCP-1, and IL-6 on the FACSCalibur.