Test Description: One male and 2-4 female F2 homozygous mice are
tested for fertility for each project. The male (-/-) mouse is
bred to two wild type female mice for 14 days. The female (-/-)
mice are bred individually with up to three different wild type
male albino mice for a period of 60 days. Pregnant females
(homozygous and wild type) are monitored daily for births,
and pup numbers are counted at birth and at post-natal days 4.
Pups are monitored daily for milk spots in their stomachs and for
growth. Special care is taken not to disturb the litter environment.