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Ovalbumin for Project: NIH-1289
Test Description: This assay evaluates a mouse's ability to produce antibodies to a specific protein antigen. The mouse is first immunized with chicken ovalbumin (OVA), a protein foreign to the mouse. Fourteen days later blood is collected and the serum is tested for the presence of antibodies to OVA by Enzyme Linked Immunosorbent Assay (ELISA). The amount of OVA-specific antibody in the serum sample is proportional to the Optical Density (OD) value generated by an instrument that scans a 96-well sample plate. Data is provided for a set of serial dilutions of each serum sample.


Results






  Ovalbumin Challenge data for Project: NIH-1289 - Results        Export Data to Spreadsheet
Mouse
Label 		Genotype Age (days) lgG1 1:100 lgG1 1:1000 lgG1 1:10000 lgG2a 1:10 lgG2a 1:100
F-95 hom 140 1.193 0.49 0.174 0.135 0.119
F-97 hom 140 2.462 0.407 0.166 2.759 0.433
F-102 hom 140 4.0 0.803 0.243 1.301 0.24
F-120 hom 140 4.0 1.153 0.3 0.488 0.167
F-93 wt 140 4.0 1.219 0.321 4.0 1.177
F-103 wt 140 0.405 0.173 0.12 0.152 0.117
 


Copyright 2005 by Lexicon Genetics Incorporated
Lexicon Phenotypic Analysis software version 05.05.00.05