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Test Description: The mononuclear cell profile is derived by staining a single sample of lysed peripheral blood from each mouse with a panel of six lineage-specific antibodies: CD45 PerCP, anti-TCRb APC, CD4 PE, CD8 FITC, pan-NK PE, and CD19 FITC. The two FITC and PE labeled antibodies stain mutually exclusive cell types. The samples are analyzed using a Becton Dickinson FACSCalibur flow cytometer with CellQuest software.


Results

TCRbeta+ T-Cells




TCRbeta- Cells






  FACS3 Analysis data for Project: NIH-1289 - Results        Export Data to Spreadsheet
Mouse
Label
Genotype Age (days) CD4+T CD8+T CD4+CD8+T CD4-CD8-T NK cells B cells Monocytes Image Thumbnail
M-73 het 106 22.96 11.83 0.07 0.52 5.58 53.41 5.63 None None
F-137 het 105 30.06 10.13 0.17 0.85 2.94 50.78 5.07 None None
M-80 hom 106 24.48 10.21 0.4 0.61 4.05 53.58 6.68 None None
M-83 hom 106 24.13 14.04 0.29 0.37 5.65 44.53 11.0 None None
F-97 hom 105 33.79 20.09 0.42 0.59 5.53 37.6 1.98 None None
F-102 hom 105 36.12 17.88 0.68 0.72 3.96 36.95 3.69 None None
M-76 wt 106 12.91 10.45 0.23 0.2 1.57 68.77 5.85 None None
F-103 wt 105 21.83 11.77 1.18 0.58 3.91 57.26 3.47 None None
 


Copyright 2005 by Lexicon Genetics Incorporated
Lexicon Phenotypic Analysis software version 05.05.00.05