Test Description: Mice were anesthetized by intraperitoneal injection
of Avertin (1.25% 2,2,2,-tribromoethanol, 20 ml/kg body weight ),
measured body length and weight, and then placed in a prone position on the
platform of the PIXImusTM Densitometer (Lunar Inc.) for a DEXA scan.
Using a Lunar PIXImus software, the Bone mineral density (BMD) and fat composition
(% fat) and total tissue mass (TTM) were determined in the regions of interest (ROI, i.e.,
whole body, vertebrae, and both femurs).