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Formalin for Project: NIH-0769
Test Description: The formalin paw test is used to assess acute and tonic nociceptive responses in mice. A metal band is placed around the left hind paw of each mouse and 20 µl of 5% formalin is subcutaneously injected in the dorsal surface of the left hind paw. Mice are individually housed in cylindrical chambers for 45 minutes. A computer records flinches per minute, total flinches for phase I (acute phase = first 8 minutes), and total flinches for phase II (tonic phase between 20 - 40 minutes) through an electromagnetic field.


Results





Results
Genotype Mouse Phase I Phase II
Wildtype: F-264 274.0 792.0
M-341 155.0 298.0
Homozygote: F-276 313.0 758.0
M-279 137.0 251.0
M-293 141.0 434.0
M-310 336.0 491.0
F-323 286.0 373.0
F-348 217.0 525.0
Genotype Mouse Phase I Phase II






  Formalin Analysis data        Export Data to Spreadsheet
Mouse Label Genotype Age (days) Chamber # Weight Interval (sec) # Flinches
F-276 hom 92 0 0.0 1200.0 43.0
F-276 hom 92 0 0.0 1680.0 49.0
F-276 hom 92 0 0.0 1020.0 28.0
F-276 hom 92 0 0.0 720.0 2.0
F-276 hom 92 0 0.0 360.0 45.0
F-276 hom 92 0 0.0 300.0 26.0
F-276 hom 92 0 0.0 1620.0 39.0
F-276 hom 92 0 0.0 2100.0 45.0
F-276 hom 92 0 0.0 2220.0 32.0
F-276 hom 92 0 0.0 1860.0 23.0
F-276 hom 92 0 0.0 240.0 54.0
F-276 hom 92 0 0.0 1560.0 46.0
F-276 hom 92 0 0.0 600.0 15.0
F-276 hom 92 0 0.0 1260.0 40.0
F-276 hom 92 0 0.0 2400.0 20.0
F-276 hom 92 0 0.0 2580.0 18.0
F-276 hom 92 0 0.0 1740.0 35.0
F-276 hom 92 0 0.0 1920.0 33.0
F-276 hom 92 0 0.0 1080.0 19.0
F-276 hom 92 0 0.0 2640.0 28.0
F-276 hom 92 0 0.0 2340.0 29.0
F-276 hom 92 0 0.0 420.0 23.0
F-276 hom 92 0 0.0 1140.0 35.0
F-276 hom 92 0 0.0 660.0 15.0
F-276 hom 92 0 0.0 540.0 6.0
F-276 hom 92 0 0.0 1380.0 41.0
F-276 hom 92 0 0.0 2280.0 26.0
F-276 hom 92 0 0.0 120.0 48.0
F-276 hom 92 0 0.0 60.0 57.0
F-276 hom 92 0 0.0 780.0 1.0
F-276 hom 92 0 0.0 1440.0 50.0
F-276 hom 92 0 0.0 960.0 39.0
F-276 hom 92 0 0.0 2460.0 13.0
F-276 hom 92 0 0.0 1320.0 50.0
F-276 hom 92 0 0.0 2160.0 46.0
F-276 hom 92 0 0.0 900.0 4.0
F-276 hom 92 0 0.0 2520.0 14.0
F-276 hom 92 0 0.0 2700.0 17.0
F-276 hom 92 0 0.0 2040.0 12.0
F-276 hom 92 0 0.0 1800.0 27.0
F-276 hom 92 0 0.0 480.0 29.0
F-276 hom 92 0 0.0 1980.0 29.0
F-276 hom 92 0 0.0 1500.0 43.0
F-276 hom 92 0 0.0 180.0 31.0
F-276 hom 92 0 0.0 840.0 1.0
M-279 hom 92 0 0.0 2100.0 3.0
M-279 hom 92 0 0.0 1080.0 1.0
M-279 hom 92 0 0.0 1440.0 22.0
M-279 hom 92 0 0.0 2400.0 0.0
M-279 hom 92 0 0.0 1740.0 21.0
M-279 hom 92 0 0.0 540.0 3.0
M-279 hom 92 0 0.0 1500.0 16.0
M-279 hom 92 0 0.0 2340.0 4.0
M-279 hom 92 0 0.0 120.0 25.0
M-279 hom 92 0 0.0 1920.0 14.0
M-279 hom 92 0 0.0 60.0 28.0
M-279 hom 92 0 0.0 1020.0 2.0
M-279 hom 92 0 0.0 2640.0 7.0
M-279 hom 92 0 0.0 480.0 1.0
M-279 hom 92 0 0.0 1320.0 14.0
M-279 hom 92 0 0.0 2280.0 10.0
M-279 hom 92 0 0.0 420.0 5.0
M-279 hom 92 0 0.0 960.0 1.0
M-279 hom 92 0 0.0 1860.0 15.0
M-279 hom 92 0 0.0 1140.0 7.0
M-279 hom 92 0 0.0 180.0 25.0
M-279 hom 92 0 0.0 1800.0 12.0
M-279 hom 92 0 0.0 360.0 11.0
M-279 hom 92 0 0.0 2460.0 1.0
M-279 hom 92 0 0.0 240.0 19.0
M-279 hom 92 0 0.0 2160.0 8.0
M-279 hom 92 0 0.0 600.0 0.0
M-279 hom 92 0 0.0 300.0 23.0
M-279 hom 92 0 0.0 1680.0 14.0
M-279 hom 92 0 0.0 1260.0 2.0
M-279 hom 92 0 0.0 2580.0 0.0
M-279 hom 92 0 0.0 840.0 1.0
M-279 hom 92 0 0.0 1980.0 8.0
M-279 hom 92 0 0.0 1200.0 7.0
M-279 hom 92 0 0.0 1620.0 22.0
M-279 hom 92 0 0.0 2520.0 1.0
M-279 hom 92 0 0.0 780.0 0.0
M-279 hom 92 0 0.0 720.0 1.0
M-279 hom 92 0 0.0 900.0 2.0
M-279 hom 92 0 0.0 2040.0 6.0
M-279 hom 92 0 0.0 1380.0 22.0
M-279 hom 92 0 0.0 2700.0 5.0
M-279 hom 92 0 0.0 1560.0 26.0
M-279 hom 92 0 0.0 2220.0 5.0
M-279 hom 92 0 0.0 660.0 4.0
M-293 hom 92 0 0.0 420.0 0.0
M-293 hom 92 0 0.0 1680.0 38.0
M-293 hom 92 0 0.0 780.0 3.0
M-293 hom 92 0 0.0 960.0 4.0
M-293 hom 92 0 0.0 1620.0 18.0
M-293 hom 92 0 0.0 2580.0 3.0
M-293 hom 92 0 0.0 1260.0 12.0
M-293 hom 92 0 0.0 2280.0 17.0
M-293 hom 92 0 0.0 2100.0 27.0
M-293 hom 92 0 0.0 1440.0 13.0
M-293 hom 92 0 0.0 840.0 2.0
M-293 hom 92 0 0.0 2040.0 24.0
M-293 hom 92 0 0.0 360.0 20.0
M-293 hom 92 0 0.0 180.0 30.0
M-293 hom 92 0 0.0 900.0 5.0
M-293 hom 92 0 0.0 1800.0 31.0
M-293 hom 92 0 0.0 1140.0 34.0
M-293 hom 92 0 0.0 1500.0 24.0
M-293 hom 92 0 0.0 2460.0 3.0
M-293 hom 92 0 0.0 1080.0 26.0
M-293 hom 92 0 0.0 2400.0 2.0
M-293 hom 92 0 0.0 480.0 0.0
M-293 hom 92 0 0.0 660.0 0.0
M-293 hom 92 0 0.0 1020.0 5.0
M-293 hom 92 0 0.0 1980.0 28.0
M-293 hom 92 0 0.0 2340.0 11.0
M-293 hom 92 0 0.0 300.0 24.0
M-293 hom 92 0 0.0 240.0 22.0
M-293 hom 92 0 0.0 1920.0 26.0
M-293 hom 92 0 0.0 600.0 7.0
M-293 hom 92 0 0.0 2700.0 5.0
M-293 hom 92 0 0.0 1200.0 20.0
M-293 hom 92 0 0.0 1380.0 21.0
M-293 hom 92 0 0.0 2520.0 2.0
M-293 hom 92 0 0.0 1860.0 21.0
M-293 hom 92 0 0.0 2220.0 4.0
M-293 hom 92 0 0.0 2160.0 26.0
M-293 hom 92 0 0.0 1560.0 21.0
M-293 hom 92 0 0.0 720.0 8.0
M-293 hom 92 0 0.0 540.0 2.0
M-293 hom 92 0 0.0 120.0 16.0
M-293 hom 92 0 0.0 60.0 29.0
M-293 hom 92 0 0.0 1740.0 29.0
M-293 hom 92 0 0.0 2640.0 1.0
M-293 hom 92 0 0.0 1320.0 21.0
 


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Lexicon Phenotypic Analysis software version 05.05.00.05