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Test Description: The formalin paw test is used to assess acute and tonic nociceptive responses in mice. A metal band is placed around the left hind paw of each mouse and 20 Ál of 5% formalin is subcutaneously injected in the dorsal surface of the left hind paw. Mice are individually housed in cylindrical chambers for 45 minutes. A computer records flinches per minute, total flinches for phase I (acute phase = first 8 minutes), and total flinches for phase II (tonic phase between 20 - 40 minutes) through an electromagnetic field.


Results





Results
Genotype Mouse Phase I Phase II
Wildtype: M-179 94.0 226.0
F-183 269.0 560.0
Homozygote: M-161 58.0 68.0
F-163 194.0 557.0
M-173 191.0 237.0
M-182 238.0 529.0
F-200 110.0 335.0
F-211 176.0 502.0
Genotype Mouse Phase I Phase II






  Formalin Analysis data        Export Data to Spreadsheet
Mouse Label Genotype Age (days) Chamber # Weight Interval (sec) # Flinches
M-161 hom 88 0 0.0 1800.0 0.0
M-161 hom 88 0 0.0 1320.0 7.0
M-161 hom 88 0 0.0 1980.0 1.0
M-161 hom 88 0 0.0 360.0 8.0
M-161 hom 88 0 0.0 2640.0 4.0
M-161 hom 88 0 0.0 660.0 22.0
M-161 hom 88 0 0.0 1080.0 1.0
M-161 hom 88 0 0.0 1260.0 4.0
M-161 hom 88 0 0.0 780.0 6.0
M-161 hom 88 0 0.0 240.0 4.0
M-161 hom 88 0 0.0 1200.0 6.0
M-161 hom 88 0 0.0 1140.0 5.0
M-161 hom 88 0 0.0 2280.0 0.0
M-161 hom 88 0 0.0 1560.0 1.0
M-161 hom 88 0 0.0 960.0 16.0
M-161 hom 88 0 0.0 1680.0 0.0
M-161 hom 88 0 0.0 60.0 8.0
M-161 hom 88 0 0.0 2520.0 6.0
M-161 hom 88 0 0.0 300.0 4.0
M-161 hom 88 0 0.0 720.0 19.0
M-161 hom 88 0 0.0 2040.0 4.0
M-161 hom 88 0 0.0 1380.0 1.0
M-161 hom 88 0 0.0 2700.0 5.0
M-161 hom 88 0 0.0 2100.0 10.0
M-161 hom 88 0 0.0 1620.0 0.0
M-161 hom 88 0 0.0 2460.0 6.0
M-161 hom 88 0 0.0 1740.0 5.0
M-161 hom 88 0 0.0 2160.0 16.0
M-161 hom 88 0 0.0 2400.0 1.0
M-161 hom 88 0 0.0 600.0 3.0
M-161 hom 88 0 0.0 2220.0 1.0
M-161 hom 88 0 0.0 420.0 0.0
M-161 hom 88 0 0.0 120.0 7.0
M-161 hom 88 0 0.0 1440.0 0.0
M-161 hom 88 0 0.0 900.0 15.0
M-161 hom 88 0 0.0 2580.0 2.0
M-161 hom 88 0 0.0 480.0 20.0
M-161 hom 88 0 0.0 1920.0 5.0
M-161 hom 88 0 0.0 1500.0 6.0
M-161 hom 88 0 0.0 840.0 1.0
M-161 hom 88 0 0.0 180.0 7.0
M-161 hom 88 0 0.0 1860.0 0.0
M-161 hom 88 0 0.0 2340.0 0.0
M-161 hom 88 0 0.0 540.0 1.0
M-161 hom 88 0 0.0 1020.0 5.0
F-163 hom 88 0 0.0 1980.0 47.0
F-163 hom 88 0 0.0 1140.0 16.0
F-163 hom 88 0 0.0 660.0 0.0
F-163 hom 88 0 0.0 480.0 10.0
F-163 hom 88 0 0.0 960.0 0.0
F-163 hom 88 0 0.0 1320.0 6.0
F-163 hom 88 0 0.0 1080.0 2.0
F-163 hom 88 0 0.0 360.0 24.0
F-163 hom 88 0 0.0 2580.0 5.0
F-163 hom 88 0 0.0 1680.0 29.0
F-163 hom 88 0 0.0 1380.0 39.0
F-163 hom 88 0 0.0 1020.0 2.0
F-163 hom 88 0 0.0 1920.0 33.0
F-163 hom 88 0 0.0 2100.0 27.0
F-163 hom 88 0 0.0 2280.0 8.0
F-163 hom 88 0 0.0 2400.0 18.0
F-163 hom 88 0 0.0 780.0 16.0
F-163 hom 88 0 0.0 840.0 8.0
F-163 hom 88 0 0.0 1200.0 22.0
F-163 hom 88 0 0.0 1500.0 36.0
F-163 hom 88 0 0.0 2460.0 24.0
F-163 hom 88 0 0.0 900.0 1.0
F-163 hom 88 0 0.0 1800.0 38.0
F-163 hom 88 0 0.0 60.0 43.0
F-163 hom 88 0 0.0 300.0 29.0
F-163 hom 88 0 0.0 1620.0 24.0
F-163 hom 88 0 0.0 2520.0 24.0
F-163 hom 88 0 0.0 180.0 25.0
F-163 hom 88 0 0.0 1740.0 29.0
F-163 hom 88 0 0.0 2220.0 21.0
F-163 hom 88 0 0.0 420.0 5.0
F-163 hom 88 0 0.0 2160.0 6.0
F-163 hom 88 0 0.0 720.0 4.0
F-163 hom 88 0 0.0 2640.0 27.0
F-163 hom 88 0 0.0 1440.0 35.0
F-163 hom 88 0 0.0 120.0 17.0
F-163 hom 88 0 0.0 2040.0 43.0
F-163 hom 88 0 0.0 240.0 41.0
F-163 hom 88 0 0.0 1860.0 40.0
F-163 hom 88 0 0.0 2700.0 2.0
F-163 hom 88 0 0.0 600.0 22.0
F-163 hom 88 0 0.0 540.0 2.0
F-163 hom 88 0 0.0 1260.0 12.0
F-163 hom 88 0 0.0 1560.0 24.0
F-163 hom 88 0 0.0 2340.0 20.0
M-173 hom 88 0 0.0 1260.0 6.0
M-173 hom 88 0 0.0 2460.0 19.0
M-173 hom 88 0 0.0 2400.0 11.0
M-173 hom 88 0 0.0 2700.0 1.0
M-173 hom 88 0 0.0 1320.0 11.0
M-173 hom 88 0 0.0 540.0 3.0
M-173 hom 88 0 0.0 360.0 31.0
M-173 hom 88 0 0.0 1200.0 16.0
M-173 hom 88 0 0.0 1560.0 10.0
M-173 hom 88 0 0.0 2040.0 24.0
M-173 hom 88 0 0.0 840.0 7.0
M-173 hom 88 0 0.0 300.0 27.0
M-173 hom 88 0 0.0 1380.0 12.0
M-173 hom 88 0 0.0 2160.0 9.0
M-173 hom 88 0 0.0 720.0 9.0
M-173 hom 88 0 0.0 240.0 18.0
M-173 hom 88 0 0.0 2520.0 10.0
M-173 hom 88 0 0.0 1680.0 2.0
M-173 hom 88 0 0.0 2100.0 19.0
M-173 hom 88 0 0.0 660.0 2.0
M-173 hom 88 0 0.0 2340.0 5.0
M-173 hom 88 0 0.0 60.0 31.0
M-173 hom 88 0 0.0 1740.0 15.0
M-173 hom 88 0 0.0 1800.0 8.0
M-173 hom 88 0 0.0 600.0 0.0
M-173 hom 88 0 0.0 1080.0 0.0
M-173 hom 88 0 0.0 420.0 16.0
M-173 hom 88 0 0.0 2640.0 0.0
M-173 hom 88 0 0.0 780.0 10.0
M-173 hom 88 0 0.0 1440.0 9.0
M-173 hom 88 0 0.0 120.0 33.0
M-173 hom 88 0 0.0 1500.0 9.0
M-173 hom 88 0 0.0 1020.0 0.0
M-173 hom 88 0 0.0 180.0 25.0
M-173 hom 88 0 0.0 900.0 0.0
M-173 hom 88 0 0.0 1620.0 5.0
M-173 hom 88 0 0.0 960.0 7.0
M-173 hom 88 0 0.0 2280.0 4.0
M-173 hom 88 0 0.0 1920.0 14.0
M-173 hom 88 0 0.0 2220.0 10.0
M-173 hom 88 0 0.0 1860.0 25.0
M-173 hom 88 0 0.0 1980.0 13.0
M-173 hom 88 0 0.0 2580.0 1.0
M-173 hom 88 0 0.0 1140.0 1.0
M-173 hom 88 0 0.0 480.0 10.0
 


Copyright 2005 by Lexicon Genetics Incorporated
Lexicon Phenotypic Analysis software version 05.05.00.05