Test Description: The skin fibroblast
proliferation assay uses the increase in the number of
cells in a standardized culture as a measure of relative
proliferative capacity. Primary fibroblast are
established from skin biopsies taken from wild type and
mutant mice. Duplicate or triplicate cultures of 0.05
million cells are plated and allow to grow for six days. At the end of the
culture period, the number of cells present in the culture is determined using a electronic particle counter.