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Test Description: The skin fibroblast proliferation assay uses the increase in the number of cells in a standardized culture as a measure of relative proliferative capacity. Primary fibroblast are established from skin biopsies taken from wild type and mutant mice. Duplicate or triplicate cultures of 0.05 million cells are plated and allow to grow for six days. At the end of the culture period, the number of cells present in the culture is determined using a electronic particle counter.


Results







  Skin Fibroblast Proliferation Data for Project: NIH-0778 - Results        Export Data to Spreadsheet
Mouse Label Genotype Age (days) Well 1 Well 2 Well 3
F-163 hom 127 0.741 0.648 0.0
F-200 hom 126 0.777 0.72 0.0
F-211 hom 126 0.885 0.795 0.0
F-223 hom 125 0.483 0.555 0.0
F-183 wt 127 0.597 0.585 0.0
F-216 wt 126 0.69 0.831 0.0
 


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Lexicon Phenotypic Analysis software version 05.05.00.05