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Functional Observation Battery for Project: NIH-0778
Test Description: The FOB is a series of situations applied to the animal to determine gross sensory and motor deficits. A subset of tests from the Irwin neurological screen that evaluates gross neurological function is used. In general, short-duration, tactile, olfactory, and visual stimuli are applied to the animal to determine their ability to detect and respond normally. These simple tests take approximately 10 minutes and the mouse is returned to its home cage at the end of testing.
Temperature Assay: Mouse body temperature was determined by gently inserting a small probe into the rectum and recording the temperature with a digital read-out. This process takes less than 5 seconds.


Results



Phenotypic differences may have been observed in the Functional Observation Battery tests.


Results data distribution (n, %) of abnormal (failed) test for project:NIH-0778

Functional Observational Battery
Test Description: The FOB is a series of situations applied to the animal to determine gross sensory and motor deficits. A subset of tests from the Irwin neurological screen that evaluates gross neurological function is used. In general, short-duration, tactile, olfactory, and visual stimuli are applied to the animal to determine their ability to detect and respond normally. These simple tests take approximately 10 minutes and the mouse is returned to its home cage at the end of testing.
BehaviorNew Category
Genotype
WildType
(WT, +/+)
Heterozygote
(Het, +/-,-/+)
Homozygote
(Hom, -/-)
n=8
n=0
n=8
"General health and Physical Condition
wt
het
hom
Whiskers Absent
2 (25%)
N/A
2 (25%)
Bald_Hair_Patches Present
3 (38%)
N/A
3 (38%)
Piloerection Present
0 (0%)
N/A
0 (0%)
Exophthalmus Present
3 (38%)
N/A
2 (25%)
Palpebral_Closure Present
0 (0%)
N/A
0 (0%)
Straub_Tail Present
0 (0%)
N/A
0 (0%)
Curly_Kinked_Tail Present
0 (0%)
N/A
0 (0%)
Kyphosis Present
0 (0%)
N/A
0 (0%)
Lordosis Present
0 (0%)
N/A
0 (0%)
Spontaneous Activity
wt
het
hom
Exhibits Wild Running Behavior
0 (0%)
N/A
0 (0%)
Exhibits Freezing Behavior
0 (0%)
N/A
0 (0%)
Exhibits Sniffing Behavior
0 (0%)
N/A
0 (0%)
Exhibits Licking Behavior
0 (0%)
N/A
0 (0%)
Rearing Behavior Absent
0 (0%)
N/A
0 (0%)
Exhibits Jumping Behavior
0 (0%)
N/A
0 (0%)
Defication Absent
1 (13%)
N/A
2 (25%)
Urination
0 (0%)
N/A
0 (0%)
Does not Move All Around The Cage
0 (0%)
N/A
0 (0%)
Head Bobbing Present
0 (0%)
N/A
0 (0%)
Head Flicking Present
0 (0%)
N/A
0 (0%)
Circling Present
0 (0%)
N/A
0 (0%)
Waltzing Present
0 (0%)
N/A
0 (0%)
Abnormal Gait Present
0 (0%)
N/A
0 (0%)
Retropulsion Present
0 (0%)
N/A
0 (0%)
Prancing Forelimbs Present
0 (0%)
N/A
0 (0%)
Tail Suspension Response
wt
het
hom
20 Second Tail Suspension Response - Normal Splay of Limbs
8 (100%)
N/A
8 (100%)
20 Second Tail Suspension Response - Does Not Turn Up Sides
0 (0%)
N/A
0 (0%)
20 Second Tail Suspension Response - Does Not Grab ipsilateral Leg
0 (0%)
N/A
0 (0%)
20 Second Tail Suspension Response - Does Not Grab Contralateral Leg
0 (0%)
N/A
0 (0%)
20 Second Tail Suspension Response - Hindpaw Clutch
0 (0%)
N/A
0 (0%)
20 Second Tail Suspension Response - Forepaw Clutch
0 (0%)
N/A
0 (0%)
20 Second Tail Suspension Response - Other Responses
0 (0%)
N/A
0 (0%)
* Blue color coding is set for a threshold of 10%
* Red color coding indicates het or hom value >= or <= 50% of the wildtype value.

Functional Observation Battery Comments:
  • M-165(wt): Bald patches around snout.
  • M-179(wt): Bald patches around snout.
  • F-216(wt): Decreased exploratory activity.
  • F-225(wt): Bald patches around snout.
  • M-231(wt): Large circular bald patch on top of head.
  • M-173(hom): Bald patches around snout.
  • M-182(hom): Large circular bald patch on top of head.
  • F-211(hom): Bald patches around snout. Decreased exploratory activity.
  • F-223(hom): Decreased exploratory activity.





  Functional Observational Battery Tests data for Project: NIH-0778 - Results        Export Data to Spreadsheet
Mouse
Label 		Genotype Age
(days) 		Whiskers Bald Hair
Patches 		Piloerection Exophthalmus Palpebral
Closure 		Curly
Kinked Tail 		Kyphosis Lordosis Wild
Running 		Freezing Sniffing Licking Rearing Jumping Defecation Urination Move All
Around
Cage 		Head
Bobbing 		Head
Flicking 		Circling Waltzing Abnormal
Gait 		Retropulsion Prancing
Forelimbs 		Body
Posture
Adjustment -
Normal
Splay Limbs 		Body
Posture
Adjustment
Fall Over 		Righting
Reflex 		Whisker
Orienting
Response -
Whiskers
Stop Moving 		Whisker
Orienting
Response -
Turn
Toward
Touch 		Touch
Reflex - eye 		Touch
Reflex - ear 		Touch
Reflex -
flank 		Pupil
Reflex 		Visual
Reaching 		Tail
Suspension
- Normal
Splay Limbs 		Tail
Suspension
- Turn Up
Sides 		Tail
Suspension
- Grab
ipsilateral
Leg 		Tail
Suspension
- Grab
Contralateral
Leg 		Tail
Suspension
- Hindpaw
Clutch 		Tail
Suspension
- Forepaw
Clutch 		Tail
Suspension
- Other
Responses 		Catalepsy
Response 		Olfactory
Response 		Temperature Temperature
Value 		Straub
Tail
M-161 hom 80 Yes No No No No No No No No No Yes No Yes No Yes No Yes No No No No No No No Yes No No No No No No No
F-163 hom 80 Yes No No No No No No No No No Yes No Yes No Yes No Yes No No No No No No No Yes No No No No No No No
M-167 hom 80 Yes No No No No No No No No No Yes No Yes No Yes No Yes No No No No No No No Yes No No No No No No No
M-173 hom 80 Yes Yes No No No No No No No No Yes No Yes No No No Yes No No No No No No No Yes No No No No No No No
M-182 hom 80 Yes Yes No No No No No No No No Yes No Yes No Yes No Yes No No No No No No No Yes No No No No No No No
F-200 hom 79 Yes No No Yes No No No No No No Yes No Yes No Yes No Yes No No No No No No No Yes No No No No No No No
F-211 hom 79 No Yes No No No No No No No No Yes No Yes No No No Yes No No No No No No No Yes No No No No No No No
F-223 hom 78 No No No Yes No No No No No No Yes No Yes No Yes No Yes No No No No No No No Yes No No No No No No No
M-165 wt 80 Yes Yes No No No No No No No No Yes No Yes No Yes No Yes No No No No No No No Yes No No No No No No No
M-179 wt 80 Yes Yes No No No No No No No No Yes No Yes No No No Yes No No No No No No No Yes No No No No No No No
F-183 wt 80 Yes No No No No No No No No No Yes No Yes No Yes No Yes No No No No No No No Yes No No No No No No No
F-198 wt 79 Yes No No Yes No No No No No No Yes No Yes No Yes No Yes No No No No No No No Yes No No No No No No No
M-203 wt 79 Yes No No No No No No No No No Yes No Yes No Yes No Yes No No No No No No No Yes No No No No No No No
F-216 wt 79 No No No Yes No No No No No No Yes No Yes No Yes No Yes No No No No No No No Yes No No No No No No No
F-225 wt 78 No Yes No Yes No No No No No No Yes No Yes No Yes No Yes No No No No No No No Yes No No No No No No No
M-231 wt 78 Yes No No No No No No No No No Yes No Yes No Yes No Yes No No No No No No No Yes No No No No No No No
 


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