Trace Aversive Conditioning for Project: NIH-0129 Test Description: Groups of naïve mice are trained in conditioning chambers (Med-Associates, St. Albans, Vermont) that have stainless steel rod floors through which foot shocks are delivered. For cued trace fear conditioning mice are placed in training context and left undisturbed for habituation purposes for 3 min. Then a conditioned stimulus (CS: 15 sec duration, 85 dB 3kHz) generated by tone is delivered, followed by a trace period of 10 sec and the unconditioned stimulus (US: foot shock, 2 sec, 0.35 mA). Mice are presented with 3 trials with inter-trial interval (ITI) 2 min and returned to the home cage 1 minute after the final shock.

Results

Trace Aversive Conditioning data        Mouse Label Genotype Age (days) Pre-CS Freezing Post-CS Freezing Difference CS Freezing F-56 het 87 0.0 20.0 20.0 M-87 het 85 3.33 4.44 1.11 F-90 het 85 0.0 1.11 1.11 M-92 het 85 1.11 0.0 -1.11 F-96 het 85 0.0 7.78 7.78 M-99 het 85 4.44 17.78 13.34 F-103 het 85 3.33 31.11 27.78 M-114 het 83 0.0 0.0 0.0 M-55 wt 87 0.0 5.56 5.56 M-69 wt 85 5.56 0.0 -5.56 F-95 wt 85 6.67 13.33 6.66 F-105 wt 85 0.0 27.78 27.78

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