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Open Field for Project: NIH-0617
Test Description: A large arena (40 cm x 40 cm) with infrared beams at three different levels is used to record rearing, hole poke, and locomotor activity. The animal is placed in the center and its activity is measured for 20 minutes. Data from this test is analyzed in five, 4-minute intervals. The total distance traveled (cm), vertical movement number (rearing), number of hole pokes, and the center:total distance ratio are then graphed.








Results Data
GenotypeMedian Rearing ActivityMedian Hole Poke ActivityMedian Normalized Slope**
+/+ (n=3)52.017.0-3.86
+/- (n=6)45.531.0-3.33
-/- (n=7)12.015.0-0.34
** Normalized slope is based on total distance traveled.


Genotype Mouse Label % of Total Distance Normalized
Slope
Interval 1
(4 min) 		Interval 2
(8 min) 		Interval 3
(12 min) 		Interval 4
(16 min) 		Interval 5
(20 min)
hom F-49 25.05 21.86 20.49 16.16 16.44 -2.29
hom F-84 18.13 22.15 22.68 19.08 17.96 -0.34
hom F-88 12.2 4.39 37.4 30.13 15.87 3.31
hom F-91 10.69 21.2 25.35 25.21 17.55 1.77
hom M-94 16.42 35.67 13.06 18.17 16.69 -1.7
hom M-95 4.66 31.19 25.4 19.26 19.49 1.77
hom F-99 27.66 30.95 18.73 18.44 4.22 -5.94
(hom)   Slope  Median:   -0.34
Mean:   -0.49
wt M-39 18.61 27.1 23.02 19.14 12.13 -2.09
wt F-51 35.97 23.34 17.14 15.75 7.81 -6.39
wt F-82 27.86 23.3 19.31 18.66 10.88 -3.86
(wt)   Slope  Median:   -3.86
Mean:   -4.11
het M-38 25.94 25.7 17.34 15.58 15.44 -3.11
het M-45 24.15 23.15 23.42 15.69 13.59 -2.86
het M-46 26.5 23.69 21.97 14.46 13.38 -3.55
het F-48 36.25 24.23 14.98 15.58 8.97 -6.32
het F-50 41.62 29.69 14.85 8.19 5.65 -9.34
het M-55 25.71 26.88 14.11 16.73 16.56 -2.84
(het)   Slope  Median:   -3.33
Mean:   -4.67




  Openfield New Analysis data for Project: NIH-0617 - Results        Export Data to Spreadsheet
Mouse Label Genotype Age (days) SAMPLE SAMP DUR TOTAL DIST VACTV VMOVNO V TIME STRNO CTR DIST PSA PRI SAMP DUR PRI SAMP AUX SAMP DUR AUX SAMP
M-38 het 91 1 240.0 531 5 2 2.4 28 62 P 4 5 0 0
M-38 het 91 1 240.0 0 0 0.0 P 4 5 0 0
M-38 het 91 2 240.0 10 5 6.2 P 4 5 0 0
M-38 het 91 2 240.0 526 17 7 8.1 38 177 P 4 5 0 0
M-38 het 91 3 240.0 355 29 14 14.6 34 136 P 4 5 0 0
M-38 het 91 4 240.0 319 6 2 2.0 32 168 P 4 5 0 0
M-38 het 91 5 240.0 316 14 5 7.1 33 97 P 4 5 0 0
M-38 het 91 5 240.0 5 4 3.4 P 4 5 0 0
M-38 het 91 4 240.0 11 6 6.1 P 4 5 0 0
M-38 het 91 3 240.0 8 5 4.9 P 4 5 0 0
M-45 het 91 1 240.0 8 6 2.6 P 4 5 0 0
M-45 het 91 1 240.0 725 54 26 25.1 41 132 P 4 5 0 0
M-45 het 91 2 240.0 17 14 7.1 P 4 5 0 0
M-45 het 91 2 240.0 695 47 23 21.6 43 218 P 4 5 0 0
M-45 het 91 5 240.0 408 37 15 17.1 37 131 P 4 5 0 0
M-45 het 91 5 240.0 5 3 1.3 P 4 5 0 0
M-45 het 91 4 240.0 471 21 14 9.7 37 143 P 4 5 0 0
M-45 het 91 4 240.0 29 14 20.3 P 4 5 0 0
M-45 het 91 3 240.0 703 79 26 31.4 42 146 P 4 5 0 0
M-45 het 91 3 240.0 17 13 13.3 P 4 5 0 0
M-46 het 91 1 240.0 9 6 4.3 P 4 5 0 0
M-46 het 91 1 240.0 830 15 6 6.3 40 181 P 4 5 0 0
M-46 het 91 4 240.0 0 0 0.0 P 4 5 0 0
M-46 het 91 5 240.0 419 53 16 22.7 32 126 P 4 5 0 0
M-46 het 91 5 240.0 6 5 3.0 P 4 5 0 0
M-46 het 91 4 240.0 453 31 13 16.5 39 183 P 4 5 0 0
M-46 het 91 2 240.0 5 4 1.7 P 4 5 0 0
M-46 het 91 3 240.0 688 34 15 13.5 38 276 P 4 5 0 0
M-46 het 91 3 240.0 27 18 12.8 P 4 5 0 0
M-46 het 91 2 240.0 742 25 12 10.1 36 231 P 4 5 0 0
F-48 het 91 1 240.0 10 8 5.3 P 4 5 0 0
F-48 het 91 1 240.0 784 35 14 16.4 40 219 P 4 5 0 0
F-48 het 91 5 240.0 194 4 4 1.6 29 108 P 4 5 0 0
F-48 het 91 5 240.0 6 7 4.8 P 4 5 0 0
F-48 het 91 4 240.0 337 23 8 10.2 27 157 P 4 5 0 0
F-48 het 91 4 240.0 10 6 4.1 P 4 5 0 0
F-48 het 91 3 240.0 324 12 4 5.4 41 187 P 4 5 0 0
F-48 het 91 3 240.0 12 11 7.2 P 4 5 0 0
F-48 het 91 2 240.0 524 30 11 12.1 42 220 P 4 5 0 0
F-48 het 91 2 240.0 13 11 5.0 P 4 5 0 0
F-50 het 91 1 240.0 3 3 0.5 P 4 5 0 0
F-50 het 91 1 240.0 869 46 22 24.5 27 83 P 4 5 0 0
F-50 het 91 5 240.0 4 3 2.1 P 4 5 0 0
F-50 het 91 5 240.0 118 11 4 6.9 25 16 P 4 5 0 0
F-50 het 91 4 240.0 171 10 5 7.0 32 23 P 4 5 0 0
F-50 het 91 4 240.0 0 0 0.0 P 4 5 0 0
F-50 het 91 3 240.0 3 2 2.2 P 4 5 0 0
F-50 het 91 3 240.0 310 15 8 15.4 31 64 P 4 5 0 0
F-50 het 91 2 240.0 620 24 11 20.0 31 119 P 4 5 0 0
F-50 het 91 2 240.0 18 17 11.3 P 4 5 0 0
M-55 het 91 1 240.0 2 2 0.2 P 4 5 0 0
M-55 het 91 1 240.0 461 13 8 4.6 37 57 P 4 5 0 0
M-55 het 91 5 240.0 297 15 10 7.3 38 40 P 4 5 0 0
M-55 het 91 5 240.0 3 3 1.2 P 4 5 0 0
M-55 het 91 4 240.0 300 14 10 7.8 35 53 P 4 5 0 0
M-55 het 91 4 240.0 5 5 3.6 P 4 5 0 0
M-55 het 91 3 240.0 253 9 5 4.3 30 75 P 4 5 0 0
M-55 het 91 3 240.0 9 6 5.8 P 4 5 0 0
M-55 het 91 2 240.0 482 14 6 5.5 38 127 P 4 5 0 0
M-55 het 91 2 240.0 20 13 9.9 P 4 5 0 0
F-49 hom 91 1 240.0 8 8 3.5 P 4 5 0 0
F-49 hom 91 4 240.0 638 40 19 12.4 41 278 P 4 5 0 0
F-49 hom 91 5 240.0 649 44 27 19.8 46 303 P 4 5 0 0
F-49 hom 91 5 240.0 12 10 10.3 P 4 5 0 0
F-49 hom 91 4 240.0 4 2 2.1 P 4 5 0 0
F-49 hom 91 3 240.0 809 31 19 16.7 45 322 P 4 5 0 0
F-49 hom 91 3 240.0 9 9 3.2 P 4 5 0 0
F-49 hom 91 2 240.0 13 13 5.1 P 4 5 0 0
F-49 hom 91 2 240.0 863 47 27 21.3 43 422 P 4 5 0 0
F-49 hom 91 1 240.0 989 30 19 14.2 42 408 P 4 5 0 0
F-84 hom 85 1 240.0 0 0 0.0 P 4 5 0 0
F-84 hom 85 1 240.0 761 45 24 29.0 42 49 P 4 5 0 0
F-84 hom 85 5 240.0 754 57 15 41.9 40 220 P 4 5 0 0
F-84 hom 85 5 240.0 12 11 4.7 P 4 5 0 0
F-84 hom 85 4 240.0 801 38 16 27.1 44 218 P 4 5 0 0
F-84 hom 85 4 240.0 18 12 8.9 P 4 5 0 0
F-84 hom 85 3 240.0 952 56 27 38.8 40 137 P 4 5 0 0
F-84 hom 85 3 240.0 1 1 0.6 P 4 5 0 0
F-84 hom 85 2 240.0 930 45 17 29.1 45 158 P 4 5 0 0
F-84 hom 85 2 240.0 22 11 14.4 P 4 5 0 0
F-88 hom 84 1 240.0 1 1 0.0 P 4 5 0 0
F-88 hom 84 2 240.0 0 0 0.0 P 4 5 0 0
F-88 hom 84 5 240.0 177 0 0 0.0 24 9 P 4 5 0 0
F-88 hom 84 5 240.0 3 2 2.4 P 4 5 0 0
F-88 hom 84 4 240.0 336 0 0 0.0 35 57 P 4 5 0 0
F-88 hom 84 4 240.0 7 7 2.7 P 4 5 0 0
F-88 hom 84 3 240.0 417 0 0 0.0 35 32 P 4 5 0 0
F-88 hom 84 3 240.0 7 5 4.9 P 4 5 0 0
F-88 hom 84 1 240.0 136 0 0 0.0 28 28 P 4 5 0 0
F-88 hom 84 2 240.0 49 0 0 0.0 26 2 P 4 5 0 0
F-91 hom 84 1 240.0 229 0 0 0.0 40 30 P 4 5 0 0
F-91 hom 84 1 240.0 5 4 0.8 P 4 5 0 0
F-91 hom 84 5 240.0 0 0 0.0 P 4 5 0 0
F-91 hom 84 5 240.0 376 0 0 0.0 33 124 P 4 5 0 0
F-91 hom 84 4 240.0 540 0 0 0.0 38 144 P 4 5 0 0
F-91 hom 84 4 240.0 8 7 3.0 P 4 5 0 0
F-91 hom 84 3 240.0 543 0 0 0.0 39 123 P 4 5 0 0
F-91 hom 84 3 240.0 6 6 3.1 P 4 5 0 0
F-91 hom 84 2 240.0 7 6 3.6 P 4 5 0 0
F-91 hom 84 2 240.0 454 0 0 0.0 38 29 P 4 5 0 0
M-94 hom 86 1 240.0 2 2 0.6 P 4 5 0 0
M-94 hom 86 1 240.0 244 0 0 0.0 28 26 P 4 5 0 0
M-94 hom 86 5 240.0 248 17 9 9.6 23 6 P 4 5 0 0
M-94 hom 86 5 240.0 0 0 0.0 P 4 5 0 0
M-94 hom 86 4 240.0 270 3 1 1.4 26 12 P 4 5 0 0
M-94 hom 86 4 240.0 3 1 0.4 P 4 5 0 0
M-94 hom 86 3 240.0 194 1 1 0.1 27 19 P 4 5 0 0
M-94 hom 86 3 240.0 0 0 0.0 P 4 5 0 0
M-94 hom 86 2 240.0 3 3 1.1 P 4 5 0 0
M-94 hom 86 2 240.0 530 5 1 1.0 30 33 P 4 5 0 0
M-95 hom 86 1 240.0 2 1 0.2 P 4 5 0 0
M-95 hom 86 1 240.0 82 2 1 0.1 26 47 P 4 5 0 0
M-95 hom 86 5 240.0 343 4 3 2.0 31 99 P 4 5 0 0
M-95 hom 86 5 240.0 2 2 0.9 P 4 5 0 0
M-95 hom 86 4 240.0 339 7 4 3.9 42 62 P 4 5 0 0
M-95 hom 86 4 240.0 3 2 0.5 P 4 5 0 0
M-95 hom 86 3 240.0 447 15 6 7.2 35 67 P 4 5 0 0
M-95 hom 86 3 240.0 13 9 6.2 P 4 5 0 0
M-95 hom 86 2 240.0 549 7 7 3.3 45 20 P 4 5 0 0
M-95 hom 86 2 240.0 0 0 0.0 P 4 5 0 0
F-99 hom 86 1 240.0 0 0 0.0 P 4 5 0 0
F-99 hom 86 1 240.0 564 2 1 0.5 30 12 P 4 5 0 0
F-99 hom 86 5 240.0 86 0 0 0.0 13 2 P 4 5 0 0
F-99 hom 86 5 240.0 0 0 0.0 P 4 5 0 0
F-99 hom 86 4 240.0 376 9 5 4.9 16 49 P 4 5 0 0
F-99 hom 86 4 240.0 0 0 0.0 P 4 5 0 0
F-99 hom 86 3 240.0 382 12 4 6.3 34 43 P 4 5 0 0
F-99 hom 86 3 240.0 2 2 1.1 P 4 5 0 0
F-99 hom 86 2 240.0 4 3 2.6 P 4 5 0 0
F-99 hom 86 2 240.0 631 0 0 0.0 34 28 P 4 5 0 0
M-39 wt 91 1 240.0 3 2 1.3 P 4 5 0 0
M-39 wt 91 1 240.0 465 20 11 7.4 37 73 P 4 5 0 0
M-39 wt 91 5 240.0 303 12 7 3.8 34 128 P 4 5 0 0
M-39 wt 91 5 240.0 9 6 2.2 P 4 5 0 0
M-39 wt 91 4 240.0 478 6 6 2.9 37 219 P 4 5 0 0
M-39 wt 91 4 240.0 14 9 7.5 P 4 5 0 0
M-39 wt 91 3 240.0 15 9 5.4 P 4 5 0 0
M-39 wt 91 3 240.0 575 22 17 10.7 37 247 P 4 5 0 0
M-39 wt 91 2 240.0 677 38 22 18.2 38 169 P 4 5 0 0
M-39 wt 91 2 240.0 9 7 3.5 P 4 5 0 0
F-51 wt 91 1 240.0 10 8 4.7 P 4 5 0 0
F-51 wt 91 2 240.0 7 5 4.1 P 4 5 0 0
F-51 wt 91 1 240.0 806 38 21 15.6 38 201 P 4 5 0 0
F-51 wt 91 3 240.0 5 4 2.4 P 4 5 0 0
F-51 wt 91 3 240.0 384 6 5 2.4 35 172 P 4 5 0 0
F-51 wt 91 5 240.0 0 0 0.0 P 4 5 0 0
F-51 wt 91 5 240.0 175 5 3 2.2 23 71 P 4 5 0 0
F-51 wt 91 4 240.0 353 13 7 4.2 29 102 P 4 5 0 0
F-51 wt 91 4 240.0 0 0 0.0 P 4 5 0 0
F-51 wt 91 2 240.0 523 33 16 10.1 31 181 P 4 5 0 0
F-82 wt 85 1 240.0 2 2 0.7 P 4 5 0 0
F-82 wt 85 2 240.0 3 3 1.8 P 4 5 0 0
F-82 wt 85 4 240.0 6 5 3.6 P 4 5 0 0
F-82 wt 85 5 240.0 267 9 6 3.9 37 73 P 4 5 0 0
F-82 wt 85 5 240.0 0 0 0.0 P 4 5 0 0
F-82 wt 85 3 240.0 0 0 0.0 P 4 5 0 0
F-82 wt 85 4 240.0 458 26 9 9.7 38 158 P 4 5 0 0
F-82 wt 85 3 240.0 474 15 9 6.4 44 192 P 4 5 0 0
F-82 wt 85 2 240.0 572 16 10 8.6 39 175 P 4 5 0 0
F-82 wt 85 1 240.0 684 13 8 8.2 45 218 P 4 5 0 0
 


Copyright 2005 by Lexicon Genetics Incorporated
Lexicon Phenotypic Analysis software version 05.05.00.05