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FACS 2 for Project: NIH-0617
Test Description: The mononuclear cell profile is derived by staining a single sample of lysed peripheral blood from each mouse with a panel of six lineage-specific antibodies: CD45 PerCP, anti-TCRb APC, CD4 PE, CD8 FITC, pan-NK PE, and CD19 FITC. The two FITC and PE labeled antibodies stain mutually exclusive cell types. The samples are analyzed using a Becton Dickinson FACSCalibur flow cytometer with CellQuest software.


Results

TCRbeta+ T-Cells




TCRbeta- Cells




  FACS Analysis data for Project: NIH-0617 - Results        Export Data to Spreadsheet
Mouse
Label 		Genotype Age (days) CD4+T CD8+T CD4+CD8+T CD4-CD8-T NK cells B cells Monocytes Image Thumbnail
M-38 het 113 24.85 14.31 1.35 0.85 7.91 46.0 4.75 None None
F-48 het 113 21.85 17.77 1.21 0.78 4.83 51.27 2.33 None None
F-49 hom 113 28.01 19.26 1.58 0.65 6.04 42.81 1.65 None None
M-94 hom 106 38.1 19.23 0.25 1.3 6.74 20.22 14.16 None None
M-95 hom 106 35.17 18.0 0.27 1.35 7.0 32.3 5.93 None None
M-39 wt 113 18.74 9.82 1.61 0.95 6.82 59.31 2.74 None None
F-51 wt 113 32.31 10.74 3.35 0.7 3.97 47.18 1.75 None None
F-82 wt 113 17.96 13.6 2.16 1.23 9.15 48.28 7.64 None None
M-96 wt 106 20.55 13.2 1.0 0.87 5.83 50.49 8.07 None None
 


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