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Acute Phase Response for Project: NIH-1841
Test Description: Bacterial lipopolysaccharide (LPS) is an endotoxin, a potent inducer of the acute phase response and systemic inflammation. The mice will:
  • Be injected intraperitoneally (i.p.) with a sublethal dose of LPS in 200 µL sterile saline using a 26 gauge needle.
  • The dose will be based on the average weight of the mice tested at 1 µg/g body weight 3 hours after injection, a 100ul blood sample will be taken and analyzed for the presence of TNFα, MCP-1, and IL-6 on the FACSCalibur.

Assay-Specific Summary :NIH-1841
Results
Acute Phase Response: The (+/-) mice exhibited a decreased median serum IL-6 response and slightly decreased median serum TNF-alpha and MCP-1 responses to LPS challenge when compared with those of their (+/+) littermates.

Results



  Acute Phase Response data for Project: NIH-1841 - Results Export Data to Spreadsheet
Mouse
Label 		Genotype Age (days) TNF-a MCP-1 IL-6
M-104 het 112 417.4 30557.8 3675.2
M-107 het 112 377.5 3143.9 262.8
M-115 het 111 1212.6 48126.2 9946.6
M-124 het 111 1130.0 42521.8 9608.7
M-79 wt 114 0.0 0.0 134.0
M-92 wt 113 1856.0 53443.3 16507.9
M-108 wt 112 2245.5 77299.2 22860.7
M-116 wt 111 1205.0 57567.4 12282.2
 

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