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Test Description: This assay evaluates a mouse's ability to produce antibodies to a specific protein antigen. The mouse is first immunized with chicken ovalbumin (OVA), a protein foreign to the mouse. Fourteen days later blood is collected and the serum is tested for the presence of antibodies to OVA by Enzyme Linked Immunosorbent Assay (ELISA). The amount of OVA-specific antibody in the serum sample is proportional to the Optical Density (OD) value generated by an instrument that scans a 96-well sample plate. Data is provided for a set of serial dilutions of each serum sample.

Assay-Specific Summary :NIH-1841
Results
Ovalbumin: No notable difference.

Results




  Ovalbumin Challenge data for Project: NIH-1841 - Results Export Data to Spreadsheet
Mouse
Label
Genotype Age (days) lgG1 1:100 lgG1 1:1000 lgG1 1:10000 lgG2a 1:10 lgG2a 1:100
F-83 het 129 4.0 1.672 0.256 4.0 2.427
F-91 het 129 1.685 0.512 0.086 1.354 0.277
F-95 het 128 4.0 1.224 0.792 3.441 1.238
F-130 het 125 4.0 1.519 0.248 0.614 0.127
F-102 wt 128 4.0 1.203 0.17 1.226 0.281
F-121 wt 126 2.48 0.439 0.101 1.249 0.196
 

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