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FACS 3 for Project: NIH-1841
Test Description: The mononuclear cell profile is derived by staining a single sample of lysed peripheral blood from each mouse with a panel of six lineage-specific antibodies: CD45 PerCP, anti-TCRb APC, CD4 PE, CD8 FITC, pan-NK PE, and CD19 FITC. The two FITC and PE labeled antibodies stain mutually exclusive cell types. The samples are analyzed using a Becton Dickinson FACSCalibur flow cytometer with CellQuest software.

Assay-Specific Summary :NIH-1841
Results
FACS: No notable difference.

Results

TCRbeta+ T-Cells




TCRbeta- Cells


  FACS3 Analysis data for Project: NIH-1841 - Results Export Data to Spreadsheet
Mouse
Label 		Genotype Age (days) CD4+T CD8+T CD4+CD8+T CD4-CD8-T NK cells B cells Monocytes
M-104 het 103 25.71 14.63 1.15 0.44 4.56 50.71 2.8
M-107 het 103 34.22 15.22 1.27 0.48 3.49 41.67 3.65
M-115 het 102 30.63 12.09 1.48 0.62 2.99 49.03 3.19
F-130 het 101 38.69 19.85 1.68 0.51 2.24 33.22 3.82
M-79 wt 105 27.78 14.56 1.69 0.59 4.52 48.01 2.86
F-121 wt 102 31.42 18.45 1.74 0.23 3.67 40.79 3.71
 

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