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Test Description: The mononuclear cell profile is derived by staining a single sample of lysed peripheral blood from each mouse with a panel of six lineage-specific antibodies: CD45 PerCP, anti-TCRb APC, CD4 PE, CD8 FITC, pan-NK PE, and CD19 FITC. The two FITC and PE labeled antibodies stain mutually exclusive cell types. The samples are analyzed using a Becton Dickinson FACSCalibur flow cytometer with CellQuest software.


Results

TCRbeta+ T-Cells




TCRbeta- Cells






  FACS3 Analysis data for Project: NIH-1790 - Results        Export Data to Spreadsheet
Mouse
Label
Genotype Age (days) CD4+T CD8+T CD4+CD8+T CD4-CD8-T NK cells B cells Monocytes Image Thumbnail
F-103 het 106 27.71 15.27 1.86 0.46 7.63 42.92 4.17 None None
M-129 het 101 26.3 16.19 0.84 0.47 2.88 49.5 3.83 None None
F-115 hom 102 22.98 17.3 1.12 0.46 3.1 51.18 3.88 None None
M-122 hom 102 28.63 17.81 0.92 0.54 6.12 39.28 6.72 None None
M-127 hom 101 27.71 14.14 1.11 0.67 5.57 44.46 6.33 None None
M-135 hom 101 23.22 17.14 2.25 0.73 7.26 42.2 7.21 None None
M-143 wt 101 23.3 17.9 2.53 0.5 3.19 47.5 5.08 None None
M-150 wt 101 32.57 17.54 1.43 0.7 4.27 36.05 7.45 None None
 


Copyright 2005 by Lexicon Genetics Incorporated
Lexicon Phenotypic Analysis software version 05.05.00.05