| Gene: 1231 | Name: ADAMTS4 | Family: Protease | Subfamily: ADAM | Accession: BC027773 | GI: 20381316 |
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Gene 1231 Behavior: When compared to age- and gender-matched wild-type control mice, homozygous mutant mice fell from the rotarod at significantly slower speeds during rotarod testing, and homozygous mutant mice required a significantly lower dose of metrazol to reach the first seizure stage during metrazol testing. There were no other significant differences detected in the homozygous mutant mice when compared with age- and gender-matched wild-type control mice. ES cells derived from the 129/OlaHsd mouse substrain were used to generate chimeric mice. F1 mice were generated by breeding with C57BL/6 females. F2 homozygous mutant mice were produced by intercrossing F1 heterozygous males and females. Wild-type control mice and homozygous mutant mice were evaluated by the following examinations or tests:
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Gene
1231 Taqman Summary: Moderate levels of RNA transcripts are detectable in eye, Harderian glands, lung, pancreas, kidney, spleen, thymus, lymph nodes, bone marrow, skin, gallbladder, urinary bladder, pituitary gland, adrenal gland, salivary gland, skeletal muscle, tongue, stomach, small intestine, cecum, colon, testis, epididymis, seminal vesicle, coagulating gland, prostate gland, and white fat. Lower levels of RNA transcripts are also detectable in heart. No RNA transcripts are detectable in liver. LacZ Summary:
Spinal cord Eyes Bone Marrow Smear Tongue Skin Ear, external Male Reproductive Systems Female Reproductive Systems Oviduct/Uterus Vagina/Cervix No Expression: |
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Gene
1231 There were no significant
differences detected in the homozygous mutant mice when compared with age-
and gender-matched wild-type control mice. The following mice were
necropsied. Body weight, body length, and organ weights were obtained, and
gross pathological findings were recorded. 49 Day Cohort Mouse ID numbers
were as follows: Necropsy Findings: There were no genotype-related or
biologically significant differences noted between mutant and wild-type
control mice for any of the parameters evaluated at necropsy. Incidental
lesions may have been present in some tissues. These findings were
considered to represent background lesions occasionally seen in this strain
of mice, lesions due to spontaneous disease, age-related lesions, and/or
lesions of a nonspecific etiology. They were not considered to be
related to genotype. Body and Organ Weight Findings: Differences in body length, body
weight, organ weights, and/or organ weight to body weight ratios were present
between individual mice. The variability between mice usually fell
within our historical reference ranges and was not correlated with
genotype. Thymus to body weight ratios were slightly decreased in all
homozygous mutant females when compared to age- and gender-matched wild-type
control mice but remained within the historical reference range. |
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Gene
1231 There were no significant differences detected in the homozygous mutant mice when compared with age- and gender-matched wild-type control mice. Tissues from the following mice were evaluated histologically. 49 Day Cohort Mouse ID numbers
were as follows: The following tissues were
examined and considered to have no genotype-related abnormality: brain,
pituitary gland, ears, nasal cavity, salivary glands, oral cavity, lymph
nodes, aorta, lungs, gallbladder, pancreas, spleen, kidneys, urinary bladder,
stomach, small and large intestines, larynx, esophagus, trachea, thyroid
gland, thymus gland, tongue, skeletal muscle, sciatic nerve, mammary glands,
vertebrae, spinal cord, bone (skull, sternum, femur, tibia, and stifle
joint), reproductive tract (including gonads), eyes, Harderian glands,
integumentary system (skin and either clitoral or preputial glands), and bone
marrow. Bone marrow was examined in
sections of sternum, vertebrae, and/or femur and tibia. Marrow cellularity,
myeloid:erythroid (M:E) ratio, myeloid and erythroid maturation sequences,
and numbers of megakaryocytes were evaluated. Incidental lesions were present
in some tissues. These findings were considered to represent background
lesions occasionally seen in this strain of mice, lesions due to spontaneous
disease, age-related lesions, and/or lesions of a nonspecific
etiology. They were not considered to be genotype related. |
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Gene
1231 There were no significant
differences in the homozygous mutant mice when compared with age- and
gender-matched wild-type control mice. Blood samples from the following
mice were evaluated by a complete blood count and differential cell count. 49 Day Cohort Mouse ID numbers
are as follows: |
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Gene
1231 There were no significant
differences in the homozygous mutant mice when compared with age- and
gender-matched wild-type control mice. Serum samples from the following
mice were evaluated by a clinical chemistry panel. 49 Day Cohort Mouse ID numbers
are as follows: |
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Gene
1231 There were no significant differences detected in the homozygous mutant mice when compared with age- and gender-matched wild-type control mice. The following mice were evaluated by dual-energy x-ray absorptiometry. 49 Day Cohort Mouse ID numbers
were as follows: Densitometric
Findings: Incidental
densitometric differences may have been present between some mice. For
example, fat % was high in one 300 day homozygous mutant female (237722).
These findings were considered to represent background differences
occasionally seen in this strain of mice, differences due to spontaneous
disease, age-related changes, differences due to procedural artifacts, and/or
differences of a nonspecific etiology. They were not considered to be
genotype related. |
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Gene
1231 There were no significant
differences detected in the homozygous mutant mice when compared with age-
and gender-matched wild-type control mice. The following mice were evaluated
by physical examination. 49 Day Cohort Mouse ID numbers
were as follows: Individual homozygous mutant mice had only occasional minor differences in observed physical features compared to wild-type control mice. These findings were considered to represent individual variability, background features occasionally seen in this strain of mice, findings due to spontaneous disease, age-related findings, and/or findings of a nonspecific etiology. However, none of these differences was regarded as biologically significant or genotype related. |
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Gene
1231 There were no significant
differences detected in the homozygous mutant mice when compared with age-
and gender-matched wild-type control mice. Body weights and body lengths
were measured for mice at 49, 90, 180, and 300 days of age. 49 Day Cohort Mouse ID numbers
are as follows: Differences in body length and body weight were present between individual mice. The variability between mice usually fell within our historical reference ranges and was not correlated with genotype. |
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Gene 1231
Homozygous
mutant and wild-type control mice were evaluated for phenotypic changes by
testing on seven behavioral tasks: Open field test, Tail suspension
test, Rotarod test, Startle response/PPI test, Tail flick test, Hot plate
test, and Metrazol test. Mouse ID numbers are as follows for the N1 generation: Behavior Findings: When
compared to age- and gender-matched wild-type control mice, homozygous mutant
mice required a significantly lower dose of metrazol to reach the first
seizure stage during metrazol testing, indicating a possible higher
propensity towards seizure phenotype. There
were no other genotype-related differences noted between homozygous mutant
and wild-type control mice for any other parameters evaluated during behavior
testing. |
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Gene 1231 Three homozygous mutant mice of each gender were set up in a fertility mating one on one with each other at seven to ten weeks of age. The number of pups born from three litters was recorded. Three weeks later, the live pups were counted and weaned. Mouse ID numbers are as follows: 3 homozygous mutant females (248353, 255275, 255283)
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