Gene: 1128 | Name: Cacng3 | Family: Channel | Subfamily: Calcium | Accession: AJ272044 | GI: 7452993 |
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Gene 1128
Summary of Phenotypic Analysis
There were no significant differences detected in the homozygous mutant mice
when compared with age- and gender-matched wild-type control mice.
ES cells derived from the 129/OlaHsd mouse substrain were used to generate
chimeric mice. F1 mice were generated by breeding with C57BL/6 females. F2
homozygous mutant mice were produced by intercrossing F1 heterozygous males and
females.
Wild-type control mice and homozygous mutant mice were evaluated by the following examinations or tests:
Gene 1128
Expression
Summary
Taqman Summary:
The highest levels of RNA
transcripts are detectable in cortex, subcortical region and olfactory bulb.
Moderate levels of RNA transcripts are detectable in: whole brain, cerebellum, brainstem, spinal cord, eye and adrenal gland.
Lower levels of RNA transcripts are also detectable in: spleen, lymph nodes, bone marrow, skin, urinary bladder, pituitary gland, salivary gland, tongue, stomach, large intestine, coagulating gland, prostate gland and ovary.
No RNA transcripts are detectable in: Harderian glands, heart, lung, liver, pancreas, kidney, thymus, gallbladder, skeletal muscle, small intestine, cecum, testis, epididymis, seminal vesicle, uterus and white fat.
LacZ Summary:
LacZ expression was detected in several of the organs examined. Most
striking expression was detected in cerebral cortex and retina. Staining was
observed in different tissue and cell types, including smooth muscle,
epithelium and neurons.
LacZ expression was detected: brain, eyes, kidney, urinary bladder, adrenal glands, testis and prostate gland.
LacZ expression was not detected in: spinal cord, sciatic nerve, thymus, spleen, lymph nodes, bone marrow, aorta, heart, lung, liver, pancreas, kidney, thyroid gland, trachea, pituitary gland, skeletal muscle, skin, ovary, uterus and cervix.
Gene 1128
Densitometry
There were no significant differences detected in the homozygous mutant mice
when compared with age- and gender-matched wild-type control mice.
The
following mice were evaluated by dual-energy x-ray absorptiometry.
49 Day Cohort Mouse ID numbers are as follows:
3 homozygous mutant females (442072, 450908, 450909)
3 homozygous mutant males (442067, 450929, 450932)
2 wild-type control females (441729, 442053)
2 wild-type control males (450919, 450920)
Bone Mineral Density (BMD in g/cm2 ), fat % (fat percentage expressed as a percentage of body soft tissue compartment), and R-value of soft tissue were calculated from Bone Mineral Content (BMC in g), bone and tissue areas (cm2 ), and total tissue mass (g) generated by a PIXImus densitometer.
Densitometric
Findings:
Incidental densitometric differences were present between some mice. These
findings were considered to represent background differences occasionally seen
in this strain of mice, differences due to spontaneous disease, age-related
differences, and/or differences of a nonspecific etiology. They were not
considered to be genotype related.
Gene 1128
Histopathology
There were no significant differences detected in the homozygous mutant mice when compared with age- and gender-matched wild-type control mice.
Tissues
from the following mice were evaluated histologically.
49 Day Cohort Mouse ID numbers are as follows:
3 homozygous mutant females (442072, 450908, 450909)
3 homozygous mutant males (442067, 450929, 450932)
2 wild-type control females (441729, 442053)
2 wild-type control males (450919, 450920)
No Significant Abnormalities:
The following tissues were examined and considered to have no genotype-related abnormality: brain, pituitary gland, ears, nasal cavity, salivary glands, oral cavity, lymph nodes, aorta, lungs, liver, gallbladder, pancreas, spleen, kidneys, adrenal glands, urinary bladder, stomach, small and large intestines, larynx, esophagus, trachea, thyroid gland, thymus, tongue, skeletal muscle, sciatic nerve, mammary glands, vertebrae, spinal cord, bone (skull, sternum, femur, tibia, and stifle joint), reproductive tract (including gonads), eyes, Harderian glands, integumentary system (skin and either clitoral or preputial glands), and bone marrow.
Bone
marrow was examined in sections of sternum, vertebrae, and/or femur and tibia.
Marrow cellularity, myeloid:erythroid (M:E) ratio, myeloid and erythroid
maturation sequences, and numbers of megakaryocytes were evaluated.
Incidental lesions were present in some tissues. These findings were
considered to represent background lesions occasionally seen in this strain of
mice, lesions due to spontaneous disease, age-related lesions, and/or lesions
of a nonspecific etiology. They were not considered to be genotype
related.
Gene 1128
Necropsy
There were no significant differences detected in the homozygous mutant mice when compared with age- and gender-matched wild-type control mice.
The following mice were
necropsied. Body weight, body length, and organ weights were obtained, and
gross pathological findings were recorded.
49 Day Cohort Mouse ID numbers are as follows:
3 homozygous mutant females (442072, 450908, 450909)
3 homozygous mutant males (442067, 450929, 450932)
2 wild-type control females (441729, 442053)
2 wild-type control males (450919, 450920)
Mice were examined for the following observables: adrenal glands, body length, body weight, bone marrow, bone - cranium, bone - femur, bone - sternum, bone - stifle joint, bone - vertebral column, brain, cecum, colon, duodenum, epididymis - seminal vesicle, esophagus, eyes, gallbladder, general appearance, Harderian glands, heart, heart weight, ileum, jejunum, kidney weight, kidneys, liver, liver weight, lungs, lymph nodes, mesentery, ovaries, pancreas, penis, salivary glands, sciatic nerve, scrotum, skeletal muscle, skin, skinned mouse, spleen, spleen weight, stomach, testes, testes - epididymis weight, thymus, thymus weight, tongue, trachea, urinary bladder, urine, uterus, and vagina. (Gender-specific observables apply to the appropriate gender.)
Necropsy Findings:
There were no genotype-related or biologically significant differences noted between mutant and wild-type control mice for any of the parameters evaluated at necropsy. Incidental lesions were present in some tissues. These findings were considered to represent background lesions occasionally seen in this strain of mice, lesions due to spontaneous disease, age-related lesions, and/or lesions of a nonspecific etiology. They were not considered to be related to genotype.
Body and Organ Weight Findings:
Differences in body length, body weight, organ weights, and/or organ weight to body weight ratios were present between individual mice. The variability between mice usually fell within our historical reference ranges and was not correlated with genotype.
Gene 1128
Clinical Chemistry
There
were no significant differences in the homozygous mutant mice when compared
with age- and gender-matched wild-type control mice.
Serum
samples from the following mice were evaluated by a clinical chemistry panel.
49
Day Cohort Mouse ID numbers are as follows:
3 homozygous mutant females (442072, 450908, 463554)
3 homozygous mutant males (442067, 450933, 452971)
2 wild-type control females (441729, 450910)
2 wild-type control males (450919, 450920)
When compared to age- and gender-matched wild-type control mice, all
homozygous mutant males (442067, 450933, 452971) had increased levels of
cholesterol and high density lipoprotein. We are not reporting these findings
as phenotypic changes, but we present them here for your consideration.
Values
for the other various analytes evaluated were generally similar between mutant
and wild-type control mice. Although variations in clinical chemistry values
were present in some mice, they were not related to genotype and, thus, were
not considered phenotypically relevant.
Gene 1128
Hematology
There
were no significant differences in the homozygous mutant mice when compared
with age- and gender-matched wild-type control mice.
Blood
samples from the following mice were evaluated by a complete blood count and
differential cell count.
49
Day Cohort Mouse ID numbers are as follows:
3 homozygous mutant females (450899, 450908, 463555)
3 homozygous mutant males (442067, 450932, 450933)
2 wild-type control females (441729, 450906)
2 wild-type control males (450919, 450920)
Although minor variations of hematological values were present in some mice,
these changes were not related to genotype and, thus, were not considered
phenotypically relevant.
Gene 1128
Physical Examination
There were no significant
differences detected in the homozygous mutant mice when compared with age- and
gender-matched wild-type control mice.
The following mice were evaluated
by physical examination.
49 Day Cohort Mouse ID numbers are as follows:
3 homozygous mutant females (442072, 450908, 450909)
3 homozygous mutant males (442067, 450929, 450932)
2 wild-type control females (441729, 442053)
2 wild-type control males (450919, 450920)
Mice
were examined in detail as follows: anus, behavior, body shape, claws, coat -
fur, coat color - back, coat color - belly, ear - left, ear - right, eye -
left, eye - right, eye color - left, eye color - right, feces, forelimb - left,
forelimb - right, forelimb number of amputated digits - left, forelimb number
of amputated digits - right, forelimb number of digits - left, forelimb number
of digits - right, general appearance, genitals - female, genitals - male, hair
type, head shape, hindlimb - left, hindlimb - right, hindlimb number of
amputated digits - left, hindlimb number of amputated digits - right, hindlimb
number of digits - left, hindlimb number of digits - right, injuries, lesions,
limb shape, locomotor, lumps - masses, mammary glands, mice in cage,
respiration, skin appearance, snout, swelling - joints, tail, teeth color,
teeth length, urine, and whiskers. (Gender-specific observables apply to the
appropriate gender.)
Individual
homozygous mutant mice had only occasional minor differences in observed
physical features compared to wild-type control mice. These findings were
considered to represent individual variability, background features
occasionally seen in this strain of mice, findings due to spontaneous disease,
age-related findings, and/or findings of a nonspecific etiology. However, none
of these differences were regarded as biologically significant or genotype
related.