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Conplastic strains are developed by backcrossing the nuclear genome from one inbred strain into the cytoplasm of another, i.e. the mitochondrial parent is always the female parent during the backcrossing program. The strain designation is NUCLEAR GENOME-mtCYTOPLASMIC GENOME. For example, C57BL/6J-mtBALB/c is a strain with the nuclear genome of C57BL/6J and the cytoplasmic genome of BALB/c. It was developed by crossing male C57BL/6J mice with BALB/c females, followed by repeated backcrossing of female offspring to male C57BL/6J. As with congenic strains, a minimum of 10 backcross generations is required, counting the F1 generation as generation 1.
See also the Rules for Nomenclature of Mouse and Rat Strains.
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