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| Nomenclature |
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Symbol:
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Tg(tetO-COX8A/PstI*)1Ctm
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Name:
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transgene insertion 1, Carlos T Moraes
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MGI ID: |
MGI:5316004 |
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Synonyms: |
mito-PstI |
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Transgene:
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Tg(tetO-COX8A/PstI*)1Ctm
Location:
unknown
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Transgene
origin |
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Strain of Origin:
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(C57BL/6 x SJL)F1
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Transgene
description |
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Transgene
Type: | |
Transgenic (random, expressed) |
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Mutation: | |
Insertion |
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Mutation details: This transgene consists of a mammalianized version of the bacterial PstI gene positioned behind a 5' mitochondrial targeting sequence from human COX VIII (COX8a) gene under the control of the tetracycline response element (TRE) promoter. The intervening sequence 8 (IVS8) was introduced between the TRE and the mito-PstI coding sequence. Southern blots indicate reduced levels of mtDNA and accumulation of partially deleted mtDNA in mice mated with tetracycline-controlled transactivator protein expressing mice, indicating that the expressed protein is functional and induces double stranded breaks in mtDNA. Treatment with Dox prevents expression of the transgene. (J:145737)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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| References |
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Original: |
J:145737
Fukui H et al.,
"Mechanisms of formation and accumulation of mitochondrial DNA deletions in aging neurons."
Hum Mol Genet 2009 Mar 15;18(6):1028-36
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All: |
2 reference(s)
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Analysis Tools
