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| Nomenclature |
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Symbol:
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Col1a1tm1(tetO-RNAi:Rps19)Karl
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Name:
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collagen, type I, alpha 1;
targeted mutation 1, Stefan Karlsson
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MGI ID: |
MGI:5304754 |
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Synonyms: |
shRNA-B |
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Gene:
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Col1a1
Location:
Chr11:94936224-94953042 bp, + strand
Genetic Position: Chr11,
59.01 cM, cytoband D
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:179085
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Parent Cell Line:
| v6.5 (ES Cell) |
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Strain of Origin:
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(C57BL/6 x 129S4/SvJae)F1
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Mutation
description |
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Allele
Type: | |
Targeted (knock-in) |
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Mutation: | |
Insertion |
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Mutation details: RMCE on KH2 cells inserts an shRNA targeting Rsp19 behind a tetracycline responsive element and CMV promoter replacing the Pou5f1 cassette in Col1a1tm2(tetO-Pou5f1)Jae into the 3'UTR. The level of knock down by this allele following doxycycline treatment is lower compared to mice expressing Col1a1tm2(tetO-RNAi:Rps19)Karl . (J:179085)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Carrying any Col1a1 Mutation:
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64 strains or lines available |
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Notes |
The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm2(tetO-Pou5f1)Jae.
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| References |
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Original: |
J:179085
Jaako P et al.,
"Mice with ribosomal protein S19 deficiency develop bone marrow failure and symptoms like patients with Diamond-Blackfan anemia."
Blood 2011 Dec 1;118(23):6087-96
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All: |
1 reference(s)
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Analysis Tools
