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| Nomenclature |
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Symbol:
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Gt(ROSA)26Sortm3(tetO-EGFP,-RNAi:T)Bgh
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Name:
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gene trap ROSA 26, Philippe Soriano;
targeted mutation 3, Bernhard G Herrmann
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MGI ID: |
MGI:5297100 |
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Synonyms: |
KD3-T |
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Gene:
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Gt(ROSA)26Sor
Location:
Chr6:113067428-113077333 bp, - strand
Genetic Position: Chr6,
52.73 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:190628
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Parent Cell Line:
| Other (see notes) (ES Cell) |
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Strain of Origin:
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Not Specified
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Mutation
description |
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Allele
Type: | |
Targeted (knock-in) |
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Mutation: | |
Insertion |
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Mutation details: Recombinase mediated cassette exchange was used to insert a tetO, CAG regulated EGFP followed by a short hairpin RNAi specific for the Brachyury (T) gene followed by a PGK promoter into the Gt(ROSA)26Sor allele. The tetracycline silencer prevents expression of both the reporter and the shRNAi until mice are treated with doxycycline. (J:173434)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Carrying any Gt(ROSA)26Sor Mutation:
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196 strains or lines available |
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Notes |
Both C57BL/6 and G4 Es cells were used for targeting.
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| References |
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Original: |
J:173434
Vidigal JA et al.,
"An inducible RNA interference system for the functional dissection of mouse embryogenesis."
Nucleic Acids Res 2010 Jun 1;38(11):e122
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All: |
2 reference(s)
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Analysis Tools
