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| Nomenclature |
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Symbol:
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Tg(Pmch-cre)1Lowl
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Name:
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transgene insertion 1, Bradford B Lowell
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MGI ID: |
MGI:4887235 |
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Synonyms: |
MCH-Cre |
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Transgene:
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Tg(Pmch-cre)1Lowl
Location:
unknown
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Transgene
origin |
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Transgene
description |
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Transgene
Type: | |
Transgenic (Cre/Flp) |
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Mutation: | |
Insertion |
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Mutation details: Homologous recombination with EL250 cells was induced to insert a cre-polyA-FRT-Neo-FRT cassette into a BAC containing the Pmch (pro-melanin-concentrating hormone; MCH) at the Pmch translational start site with concomitant replacement of the 26 nucleotides following it. Arabinose-induced Flp recombination in bacterial cells removed the kanamycin cassette. The purified, linearized construct was injected into the pronuclei of FVB one-cell embryos. Eight lines were generated and crossed to Z/EG reporter animals. 2 lines exhibited GFP expression in the lateral hypothalamus and zona incerta, known sites of endogenous Pmch expression. Cre expression in one of these lines of characterized further. (J:167906)
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Recombinase activity
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Driver:
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Pmch
Summary of all recombinase alleles
driven by Pmch.
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MGI has not yet included tissue activity data for this allele in any anatomical systems.
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:167906
Kong D et al.,
"Glucose stimulation of hypothalamic MCH neurons involves K(ATP) channels, is modulated by UCP2, and regulates peripheral glucose homeostasis."
Cell Metab 2010 Nov 3;12(5):545-52
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All: |
1 reference(s)
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Analysis Tools
