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| Nomenclature |
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Symbol:
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Nfkbiatm1.1Pjc
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Name:
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nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha;
targeted mutation 1.1, Paul J Chiao
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MGI ID: |
MGI:4829878 |
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Synonyms: |
IkappaBalphaM, IkBaM |
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Gene:
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Nfkbia
Location:
Chr12:55489411-55492647 bp, - strand
Genetic Position: Chr12,
24.0 cM, cytoband C1-C3
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:163686
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Parent Cell Line:
| Not Specified (ES Cell) |
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Strain of Origin:
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Not Specified
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Mutation
description |
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Allele
Type: | |
Targeted (knock-in) |
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Mutations: | |
Insertion, Nucleotide substitutions |
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Mutation details: Two well defined NFKB binding sites (M1 and M2) and 4 NFKB like binding sites (M3 - M6) in the promoter region were mutated, a FLAG tag was attached to exon 1, and a floxed neo cassette was inserted in intron 1 via homologous recombination. Cre mediated recombination removed the neo cassette. The locations of and mutations in the 6 binding sites are as follows: -115 - -93, a TCC motif changed to a CCG (M1); -143 - -122, a CCC motif changed to an AGT (M2); -227 - -205, a TCT motif changed to a CAC (M3); -292 - -271, a CC motif changed to a AA (M4); -319 - -297, a GGG motif changed to a TTT (M5); -387 - -365, a CCC motif changed to a GGG (M6). Chromatin immunoprecipitation analysis confirmed the loss of kappa B enhancer function in MEFs from homozygous mice. (J:163686)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Carrying any Nfkbia Mutation:
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5 strains or lines available |
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| References |
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Original: |
J:163686
Peng B et al.,
"Defective feedback regulation of NF-kappaB underlies Sjogren's syndrome in mice with mutated kappaB enhancers of the IkappaBalpha promoter."
Proc Natl Acad Sci U S A 2010 Aug 24;107(34):15193-8
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All: |
1 reference(s)
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Analysis Tools
