Tg(Thy1-COP3/EYFP)8Gfng
Transgene Detail
|
|
| Nomenclature |
|
Symbol:
|
Tg(Thy1-COP3/EYFP)8Gfng
|
|
Name:
|
transgene insertion 8, Guoping Feng
|
|
MGI ID: |
MGI:4454791 |
|
Synonyms: |
Thy1-vChR1-EYFP Line 8, Thy1-vChR1-YFP line 8 |
|
Transgene:
|
Tg(Thy1-COP3/EYFP)8Gfng
Location:
unknown
|
|
Transgene
origin |
|
Strain of Origin:
|
(C57BL/6 x SJL)F1
|
|
Transgene
description |
|
Transgene
Type: | |
Transgenic (Reporter) |
|
Mutation: | |
Insertion |
| |
|
Mutation details: A fusion protein was created with a synthetic, mammalian codon-optimized, red-shifted channelrhodopsin-1 cDNA sequence derived from Volvox carteri (VChR1; GenBank accession number EU622855 [residues 1-300]) fused in-frame to the amino terminus of an enhanced yellow fluorescent protein sequence (EYFP). This enhanced fusion protein (VChR1::YFP) was placed downstream of the modified regulatory region of the "murine thy1.2 gene" (extending from the promoter to the intron following exon 4, excluding exon 3 and its flanking introns), and followed by a bovine growth hormone polyA signal. Mice derived from founder line 8 exhibit high EGFP expression in layer 5 pyramidal neurons at cortex, CA1, CA3 and dentate gyrus of hippocampus, thalamus, superior colliculus, inferior culliculus, brainstem, amydagala and cerebellum. (J:160659)
|
|
Phenotypes
|
View phenotypes for all genotypes (concatenated display).
|
| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
|
| References |
|
Original: |
J:160659
Feng G,
"DDS for Tg(Thy1-COP3/EYFP)8Gfng"
MGI Direct Data Submission 2010;():
|
|
All: |
1 reference(s)
|
|
|
Analysis Tools
