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| Nomenclature |
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Symbol:
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Cr2tm2.1(HLA-A)Crr
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Name:
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complement receptor 2;
targeted mutation 2.1, Michael C Carroll
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MGI ID: |
MGI:4356009 |
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Synonyms: |
Cr2deltaGFP |
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Gene:
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Cr2
Location:
Chr1:195136811-195176715 bp, - strand
Genetic Position: Chr1,
98.44 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:151991
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Parent Cell Line:
| Bruce 4 (ES Cell) |
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Strain of Origin:
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B6.Cg-Thy1a
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Mutation
description |
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Allele
Type: | |
Targeted (Reporter) |
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Mutation: | |
Insertion |
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Mutation details: Sequence encoding the transmembrane and cytoplasmic regions of the human HLA class I alpha chain was positioned in frame adjacent to exon 15 to replace the analogous region of the endogenous gene product. A floxed neomycin selection gene and an IRES-GFP followed by a stop codon were inserted downstream before exon 16. Founder mice were crossed with cre deleter mice to remove the neomycin cassette. GFP expression was detected in B cells and follicular dendritic cells. The gene fusion product's expression levels were comparable to levels of the endogenous receptor in wild-type mice. The gene fusion product retained the ability to bind the complement factor C3d but lost the ability to form a complex with CD19. (J:151991)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Carrying any Cr2 Mutation:
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2 strains or lines available |
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| References |
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Original: |
J:151991
Barrington RA et al.,
"Uncoupling CD21 and CD19 of the B-cell coreceptor."
Proc Natl Acad Sci U S A 2009 Aug 25;106(34):14490-5
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All: |
1 reference(s)
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Analysis Tools
