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| Nomenclature |
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Symbol:
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Dlg4tm2.1Grnt
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Name:
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discs, large homolog 4 (Drosophila);
targeted mutation 2.1, Seth G N Grant
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MGI ID: |
MGI:3848928 |
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Synonyms: |
PSD-95GK |
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Gene:
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Dlg4
Location:
Chr11:70017085-70045532 bp, + strand
Genetic Position: Chr11,
42.96 cM, cytoband B4
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:89778
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Parent Cell Line:
| E14TG2aIV (ES Cell) |
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Strain of Origin:
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129P2/OlaHsd
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Mutation
description |
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Allele
Type: | |
Targeted (knock-out) |
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Mutations: | |
Insertion, Intragenic deletion |
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Mutation details: A STOP codon and coding sequence for haemaglutinin (HA) epitope tag were inserted in frame before the start of the GK domain at amino acid 500. In addition, a floxed neo cassette was inserted downstream to replace sequence and this cassette was subsequently excised by Cre leaving a single loxP site after the STOP codon. Western blot and immunoprecipitation analysis confirmed the absence of protein in hippocampal extracts from homozygous mice. RT-PCR analysis indicated that a very low level of mRNA expression (less than 5% of wild-type) was present in hippocampal extracts from homozygous mice. (J:149508)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Carrying any Dlg4 Mutation:
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6 strains or lines available |
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| References |
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Original: |
J:89778
Yao WD et al.,
"Identification of PSD-95 as a regulator of dopamine-mediated synaptic and behavioral plasticity."
Neuron 2004 Feb 19;41(4):625-38
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All: |
3 reference(s)
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Analysis Tools
