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| Nomenclature |
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Symbol:
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Mcl1tm1Yen
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Name:
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myeloid cell leukemia sequence 1;
targeted mutation 1, Hsin-Fang Yang-Yen
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MGI ID: |
MGI:3837368 |
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Synonyms: |
Mcl-1SC |
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Gene:
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Mcl1
Location:
Chr3:95658721-95663176 bp, + strand
Genetic Position: Chr3,
40.74 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:146246
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Parent Cell Line:
| R1 (ES Cell) |
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Strain of Origin:
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(129X1/SvJ x 129S1/Sv)F1-Kitl+
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Mutation
description |
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Allele
Type: | |
Targeted (knock-out) |
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Mutations: | |
Insertion, Intragenic deletion |
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Mutation details: Two upstream promoter elements located at positions -87 (the SIE site) and -70 (the CRE-2 site) were targeted for mutation. The SIE and CRE-2 sites were replaced with BamHI and EcoRI restriction site, respectively, and a floxed neomycin resistance cassette was placed downstream of exon 1. Founder mice were crossed with EIIa-Cre transgenic mice to remove the neomycin cassette. Immunoblot analysis revealed gene expression was reduced by 90% in the thymus but was marginally or not at all affected in various other major organs examined, including liver, heart, lung and spleen. The altered expression was attributed to the lack of SIE and CRE-2 sites because expression was not disturbed in the thymus of mice homozygous for the same loxP insertion but with intact promoter elements. (J:146246)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Carrying any Mcl1 Mutation:
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41 strains or lines available |
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| References |
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Original: |
J:146246
Yang CY et al.,
"Promoter knock-in mutations reveal a role of Mcl-1 in thymocyte-positive selection and tissue or cell lineage-specific regulation of Mcl-1 expression."
J Immunol 2009 Mar 1;182(5):2959-68
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All: |
1 reference(s)
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Analysis Tools
