|
|
| Nomenclature |
|
Symbol:
|
Leprtm1Yli
|
|
Name:
|
leptin receptor;
targeted mutation 1, Yong Liu
|
|
MGI ID: |
MGI:3822311 |
|
Synonyms: |
Ob-RbF985/1077/1138, Y123F |
|
Gene:
|
Lepr
Location:
Chr4:101717404-101815352 bp, + strand
Genetic Position: Chr4,
46.96 cM
|
|
Mutation
origin |
|
Germline Transmission:
|
Earliest citation of germline transmission:
J:142218
|
|
Parent Cell Line:
| Not Specified (ES Cell) |
|
Strain of Origin:
|
129
|
|
Mutation
description |
|
Allele
Type: | |
Targeted (knock-in) |
|
Mutations: | |
Insertion, Nucleotide substitutions |
| |
|
Mutation details: Three tyrosine residues in exon 18 (985, 1077, 1138) were replaced with phenylalanine and a floxed neo cassette was inserted after exon 18 via homologous recombination. The floxed neo cassette was then removed by cre mediated recombination leaving behind a single loxP site. Quantitative RT-PCR indicated that expression of the long isoform in the hypothalamus of homozygotes was similar to that in wild-type mice. Replacement of these tyrosines with phenylalanines abolished leptin stimulated STAT3 phosphorylation in the hypothalamus. (J:142218)
|
|
Phenotypes
|
View phenotypes for all genotypes (concatenated display).
|
|
Disease models
|
|
| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
|
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
|
Carrying any Lepr Mutation:
|
27 strains or lines available |
|
| References |
|
Original: |
J:142218
Jiang L et al.,
"Tyrosine-dependent and -independent actions of leptin receptor in control of energy balance and glucose homeostasis."
Proc Natl Acad Sci U S A 2008 Nov 25;105(47):18619-24
|
|
All: |
1 reference(s)
|
|
Analysis Tools
