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| Nomenclature |
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Symbol:
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H2-T23tm3Cant
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Name:
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histocompatibility 2, T region locus 23;
targeted mutation 3, Harvey Cantor
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MGI ID: |
MGI:3821721 |
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Synonyms: |
Qa-1 D227K |
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Gene:
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H2-T23
Location:
Chr17:36029773-36032855 bp, - strand
Genetic Position: Chr17,
18.86 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:142087
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Parent Cell Line:
| TC1/TC-1 (ES Cell) |
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Strain of Origin:
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129S6/SvEvTac
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Mutation
description |
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Allele
Type: | |
Targeted (knock-in) |
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Mutations: | |
Insertion, Nucleotide substitutions |
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Mutation details: Exon 4 of the gene locus was targeted with a mutation that causes an aspartic acid to lysine exchange at amino acid position 227 (D227K). A floxed neomycin resistance cassette was also inserted downstream of exon 5 and was subsequently removed by crossing chimeric mice with a cre transgenic strain. The mutant protein was expressed on the surface of activated CD4+ T cells from homozygous mice at levels similar to the endogenous protein in wild-type mice. This mutation was predicted to disrupt binding to the CD8 coreceptor complex. (J:142087)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Carrying any H2-T23 Mutation:
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4 strains or lines available |
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| References |
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Original: |
J:142087
Lu L et al.,
"Regulation of CD8+ regulatory T cells: Interruption of the NKG2A-Qa-1 interaction allows robust suppressive activity and resolution of autoimmune disease."
Proc Natl Acad Sci U S A 2008 Dec 9;105(49):19420-5
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All: |
4 reference(s)
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Analysis Tools
