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| Nomenclature |
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Symbol:
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Del(7Olfr64-Hbb-ar)1Mkg
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Name:
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deletion, Chr 7, Mark Groudine 1
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MGI ID: |
MGI:3810287 |
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Synonyms: |
deltaHS-62.5-deltaLCR |
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Gene:
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Del(7Olfr64-Hbb-ar)1Mkg
Location:
unknown
Genetic Position: Chr7,
Syntenic
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:139198
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Parent Cell Line:
| BK4 (ES Cell) |
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Strain of Origin:
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129P2/OlaHsd
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Mutation
description |
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Allele
Type: | |
Targeted (knock-out) |
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Mutation: | |
Intergenic deletion |
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Mutation details: ES cells carrying the Hbb-artm1.1Mkg allele, which has most of the Hbb-ar region replaced with a loxP site, were targeted in cis with a construct that replaced the upstream HS-62.5 element with a loxP-flanked neomycin selection cassette. This substitution removed nucleotides -63238 through -59669, relative to the start site of the Hbb-y gene. Transient expression of Cre recombinase eliminated the entire genomic sequence between the newly inserted floxed neo cassette and the loxP site present from the Hbb-artm1.1Mkg allele. This deletion removes genes Olfr64, Olf65, Olf66, and almost all of the Hbb-ar region. (J:139198)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Carrying any Del(7Olfr64-Hbb-ar)1Mkg Mutation:
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0 strains or lines available |
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Notes |
Male ES cells, deltaLCR-D/S-1, were generated from male mice on a 129 background that were heterozygous for the Hbb-artm1.1Mkg allele, carried two different variants of the Hbb genes (HbbD/HbbS) and were hemizygous for the null Hprtb-m3 allele.
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| References |
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Original: |
J:139198
Bender MA et al.,
"Flanking HS-62.5 and 3' HS1, and regions upstream of the LCR, are not required for beta-globin transcription."
Blood 2006 Aug 15;108(4):1395-401
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All: |
1 reference(s)
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Analysis Tools
