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| Nomenclature |
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Symbol:
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En1tm8.1Alj
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Name:
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engrailed 1;
targeted mutation 8.1, Alexandra L Joyner
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MGI ID: |
MGI:3789091 |
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Synonyms: |
En1flox |
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Gene:
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En1
Location:
Chr1:120602418-120609296 bp, + strand
Genetic Position: Chr1,
52.74 cM, cytoband C2-qter
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:134516
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Parent Cell Line:
| W4 (ES Cell) |
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Strain of Origin:
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129S6/SvEvTac
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Mutation
description |
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Allele
Type: | |
Targeted (Floxed/Frt) |
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Mutations: | |
Insertion, Intragenic deletion |
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Mutation details: A targeting construct was designed to insert an frt-flanked neomycin cassette and loxP site upstream, and a loxP site downstream of the second exon of the targeted gene. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with Black Swiss mice (to avoid rescue of the En1 mutant phenotype by the C57BL/6 background). The resulting En1flox-neo mice were then bred to 129 mice constitutively expressing Flp recombinase to remove the Frt-flanked neo cassette, generating En1flox offspring. (J:134516)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:134516
Sgaier SK et al.,
"Genetic subdivision of the tectum and cerebellum into functionally related regions based on differential sensitivity to engrailed proteins."
Development 2007 Jun;134(12):2325-35
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All: |
7 reference(s)
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Analysis Tools
