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| Nomenclature |
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Symbol:
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Snord116tm1Uta
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Name:
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small nucleolar RNA, C/D box 116 cluster;
targeted mutation 1, Uta Francke
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MGI ID: |
MGI:3773671 |
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Synonyms: |
2-lox, 2-loxp |
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Gene:
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Snord116
Location:
unknown
Genetic Position: Chr7,
Syntenic
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:131427
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Parent Cell Line:
| Bruce 4 (ES Cell) |
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Strain of Origin:
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B6.Cg-Thy1a
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Mutation
description |
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Allele
Type: | |
Targeted (Floxed/Frt) |
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Mutation: | |
Insertion |
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Mutation details: Two individual targeting vectors were used to place a loxP site (and an Frt1-flanked PGK-neo cassette) upstream, and a loxP site (and an Frt5-flanked puromycin resistance/TK cassette) downstream of the Snord116 cluster. The upstream targeting vector was transfected into C57BL/6-derived embryonic stem ES cells, and correctly targeted ES cells were next transfected with the downstream targeting vector. Doubly targeted ES cells were then transiently transfected with an FLP expressing plasmid to remove the two selection cassettes. The resulting 2-loxP ES cells (with a single loxP site just upstream, and a single loxP site just downstream of the Snord116 cluster. (J:131427)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:131427
Ding F et al.,
"SnoRNA Snord116 (Pwcr1/MBII-85) Deletion Causes Growth Deficiency and Hyperphagia in Mice."
PLoS ONE 2008;3(3):e1709
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All: |
1 reference(s)
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Analysis Tools
