|
|
| Nomenclature |
|
Symbol:
|
Tg(CAG-SAC/EGFP)35Rang
|
|
Name:
|
transgene insertion 35, Vivek Rangnekar
|
|
MGI ID: |
MGI:3772442 |
|
Synonyms: |
SAC/35, SAC-GFP line 35, Tg(ACTB-SAC/EGFP)35Rang |
|
Transgene:
|
Tg(CAG-SAC/EGFP)35Rang
Location:
unknown
|
|
Transgene
origin |
|
Strain of Origin:
|
(C57BL/6 x C3H)F1
|
|
Transgene
description |
|
Transgene
Type: | |
Transgenic (random, expressed) |
|
Mutation: | |
Insertion |
| |
|
Mutation details: The pCA/SAC-GFP transgene was designed with a chicken beta-actin promoter (and cytomegalovirus (CMV) enhancer) upstream of a fusion gene containing a Par-4 SAC domain (Pawr gene, amino acids 137-195) cDNA fused to an enhanced green fluorescent protein (EGFP), all followed by a polyA sequence. The resulting transgenic offspring were bred to (C57BL/6 x C3H)F1 mice. Founder line 35 mice (SAC/35; found to have 6-8 copies of the transgene). Widespread expression of the transgene is observed in all tested tissues (with some differential tissue-specific regulation of transgene expression or protein stability reported). EGFP expression level is appropriate for immunoblots, but not sufficient enough for fluorescence of flow cytometry applications. (J:125633)
|
|
Phenotypes
|
View phenotypes for all genotypes (concatenated display).
|
|
Disease models
|
|
| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
|
| References |
|
Original: |
J:125633
Zhao Y et al.,
"Cancer resistance in transgenic mice expressing the SAC module of Par-4."
Cancer Res 2007 Oct 1;67(19):9276-85
|
|
All: |
1 reference(s)
|
|
Analysis Tools
