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| Nomenclature |
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Symbol:
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Lgr5tm1(cre/ERT2)Cle
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Name:
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leucine rich repeat containing G protein coupled receptor 5;
targeted mutation 1, Hans Clevers
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MGI ID: |
MGI:3764660 |
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Synonyms: |
Lgr5-cre, Lgr5-EGFP, Lgr5 EGFP-IRES-creERT2, Lgr5-EGFP-IRES-creERT2, Lgr5tm1(cre/ESR1)Cle |
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Gene:
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Lgr5
Location:
Chr10:115450311-115587780 bp, - strand
Genetic Position: Chr10,
63.51 cM
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Mutation
origin |
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Germline Transmission:
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Earliest citation of germline transmission:
J:127123
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Parent Cell Line:
| IB10/E14IB10 (ES Cell) |
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Strain of Origin:
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129P2/OlaHsd
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Mutation
description |
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Allele
Type: | |
Targeted (knock-in) |
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Mutation: | |
Insertion |
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Mutation details: An EGFP-IRES-creERT2 cassette was inserted into exon 1 of the gene along with a loxP-flanked neomycin cassette. The neomycin gene was then excised in vivo by crossing with cre-deleter strain. GFP expression is driven by the endogenous promoter. Cre expression is induced in Lgr5 expressing cells by tamoxifen treatment. (J:127123)
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Recombinase activity
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Activity in:
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alimentary system,
integumental system
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Not detected in:
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cardiovascular system,
endocrine system,
hemolymphoid system,
liver & biliary system,
muscle,
nervous system,
postnatal-other,
renal & urinary system,
reproductive system,
respiratory system,
sensory organs
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Driver:
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Lgr5
Summary of all recombinase alleles
driven by Lgr5.
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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| References |
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Original: |
J:127123
Barker N et al.,
"Identification of stem cells in small intestine and colon by marker gene Lgr5."
Nature 2007 Oct 25;449(7165):1003-7
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All: |
50 reference(s)
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Analysis Tools
