|
|
| Nomenclature |
|
Symbol:
|
Kiss1tm1Rla
|
|
Name:
|
KiSS-1 metastasis-suppressor;
targeted mutation 1, Risto Lapatto
|
|
MGI ID: |
MGI:3762755 |
|
Gene:
|
Kiss1
Location:
Chr1:133327212-133329722 bp, + strand
Genetic Position: Chr1,
57.91 cM
|
|
Mutation
origin |
|
Germline Transmission:
|
Earliest citation of germline transmission:
J:126704
|
|
Parent Cell Line:
| Not Specified (ES Cell) |
|
Strain of Origin:
|
129S
|
|
Mutation
description |
|
Allele
Type: | |
Targeted (knock-out) |
|
Mutations: | |
Insertion, Intragenic deletion |
| |
|
Mutation details: Exon 1 was replaced with a neomycin resistance cassette. Southern blotting confirmed recombination but RT-PCR of exon 2 detected a transcript from the gene. Amplification and sequencing of the transcript revealed a previously unknown exon upstream of the targeted exon that was splicing to exon 2. Because no translational start site was found in this sequence, the mutation was predicted not to produce a functional protein. This was confirmed by immunohistochemistry on the hypothalamus. (J:126704)
|
|
Phenotypes
|
View phenotypes for all genotypes (concatenated display).
|
|
Disease models
|
|
| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
|
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
|
Carrying any Kiss1 Mutation:
|
2 strains or lines available |
|
| References |
|
Original: |
J:126704
Lapatto R et al.,
"Kiss1-/- mice exhibit more variable hypogonadism than Gpr54-/- mice."
Endocrinology 2007 Oct;148(10):4927-36
|
|
All: |
1 reference(s)
|
|
Analysis Tools
