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| Nomenclature |
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Symbol:
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Tg(Ckm-APPSw)A6Lfa
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Name:
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transgene insertion A6, Frank M LaFerla
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MGI ID: |
MGI:3720796 |
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Synonyms: |
A6 line, Tg(Ckm-APP*K670N*M671L)A6Lfa |
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Transgene:
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Tg(Ckm-APPSw)A6Lfa
Location:
unknown
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Transgene
origin |
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Strain of Origin:
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C57BL/6 x SJL
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Transgene
description |
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Transgene
Type: | |
Transgenic (random, expressed) |
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Mutation: | |
Insertion |
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Mutation details: Human APP cDNA harboring the Swedish double mutation (K670N,M671L) was inserted downstream of the 1.3 kb 5' flanking sequence of the mouse muscle creatine kinase Ckm, upstream of the SV40 polyadenylation signal. The 3.8 kb construct was microinjected into pronuclei of single-cell C57Bl/6 x SJL embryos. Northern blot showed relatively high expression of the transgene in skeletal muscle of line A6 mice, approximately 12 times higher than in Tg(Ckm-APP*K670N*M671L)A2Lfa mice. Western blot showed that transgenic protein expression is ~9 times higher than in mice from line A2. (J:76338)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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| References |
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Original: |
J:76338
Sugarman MC et al.,
"Inclusion body myositis-like phenotype induced by transgenic overexpression of beta APP in skeletal muscle."
Proc Natl Acad Sci U S A 2002 Apr 30;99(9):6334-9
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All: |
5 reference(s)
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Analysis Tools
