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| Nomenclature |
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Symbol:
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Tg(DMD-APP*)18Neve
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Name:
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transgene insertion 18, Rachel L Neve
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MGI ID: |
MGI:3720650 |
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Synonyms: |
FLAGC100 |
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Transgene:
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Tg(DMD-APP*)18Neve
Location:
unknown
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Transgene
origin |
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Strain of Origin:
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(C57BL/6 x SJL)F2
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Transgene
description |
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Transgene
Type: | |
Transgenic (random, expressed) |
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Mutation: | |
Insertion |
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Mutation details: A fragment consisting composed of the sequence ot the terminal 100 amino acids of the beta-amyloid precursor protein APP gene (C100, referred to as C104 in J:105050 which states that the transgene contains the terminal 100 amino acids of APP) was inserted into a plasmid encoding the neural-specific dystrophin (DMD) promoter. The vector also contained the SV40 early splice and polyadenylation sequences. A hydrophilic 10-amino acid FLAG sequence was engineered onto the aminoterminus of the C100 fragment. The construct was injected into (C57BL/6 x SJL)F2 fertilized eggs. Six founder mice were identified, 5 showing higher expression than the identified line 2. Line 18 was chosen to represent the higher expressing transgenic lines. RT-PCR revealed expression of the FLAGC100 in brains of all transgenic lines.
(J:100978)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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Disease models
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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| References |
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Original: |
J:100978
Neve RL et al.,
"Transgenic mice expressing APP-C100 in the brain."
Neurobiol Aging 1996 Mar-Apr;17(2):191-203
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All: |
1 reference(s)
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Analysis Tools
