|
|
| Nomenclature |
|
Symbol:
|
Il4ratm2Tch
|
|
Name:
|
interleukin 4 receptor, alpha;
targeted mutation 2, Talal A Chatila
|
|
MGI ID: |
MGI:3720593 |
|
Synonyms: |
IL-4RalphaR576, Il4raR576, Q576R |
|
Gene:
|
Il4ra
Location:
Chr7:125552282-125579472 bp, + strand
Genetic Position: Chr7,
68.94 cM
|
|
Mutation
origin |
|
Germline Transmission:
|
Earliest citation of germline transmission:
J:152376
|
|
Parent Cell Line:
| RW-4 (ES Cell) |
|
Strain of Origin:
|
129X1/SvJ
|
|
Mutation
description |
|
Allele
Type: | |
Targeted (knock-in) |
|
Mutation: | |
Insertion |
| |
|
Mutation details: The targeting vector was designed (by site-directed mutagenesis) to change the alanine-guanine to guanine-alanine at basepair 1964-65 of murine IL-4Ra cDNA, corresponding to a single amino acid substitution of glutamine to arginine at codon 576 (Q576R). This also inserted a loxp-flanked PGK-neo cassette within intron 11. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre-expressing vector to remove the selection cassette (leaving a single loxp site in intron 11) and then injected into C57BL/6 blastocysts. (J:152376)
|
|
Phenotypes
|
View phenotypes for all genotypes (concatenated display).
|
|
Disease models
|
|
| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
|
| References |
|
Original: |
J:152376
Tachdjian R et al.,
"Pathogenicity of a disease-associated human IL-4 receptor allele in experimental asthma."
J Exp Med 2009 Sep 28;206(10):2191-204
|
|
All: |
1 reference(s)
|
|
Analysis Tools
