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| Nomenclature |
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Symbol:
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Tg(Thy1-PSEN1*A246E)2Vln
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Name:
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transgene insertion 2, Fred Van Leuven
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MGI ID: |
MGI:3717624 |
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Synonyms: |
Ps1 A246E, Ps1/A246E, PS1[A246E], PS1mut |
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Transgene:
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Tg(Thy1-PSEN1*A246E)2Vln
Location:
unknown
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Transgene
origin |
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Transgene
description |
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Transgene
Type: | |
Transgenic (random, expressed) |
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Mutation: | |
Insertion |
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Mutation details: cDNA encoding the human PSEN1 sequence containing the A246E mutation was ligated into the mouse Thy1 gene. In the final construct, coding sequences and introns 1 and 2 of the Thy1 gene are replaced by the mutant PSEN1 sequence, with the 5' neuron-specific control sequences of Thy1 intact. Linearized minigenes are microinjected into prenuclear FVB/N embryos. Mutant PSEN1 mRNA and protein are detected in total brain extracts by Northern and Western blotting, respectively. (J:64209, J:100968)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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Notes |
J:100968 discusses generation of trangenic PSEN1[A246E] mice, referencing J:64209 as one paper on the method. Since the transgenic mice are apparently identical, it was assumed that new transgenic mice were not created, but mice are actually the ones referenced in J:64209. -rbabiuk 8/9/2007
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| References |
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Original: |
J:64209
Dewachter I et al.,
"Aging increased amyloid peptide and caused amyloid plaques in brain of old APP/V717I transgenic mice by a different mechanism than mutant presenilin1."
J Neurosci 2000 Sep 1;20(17):6452-8
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All: |
11 reference(s)
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Analysis Tools
