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| Nomenclature |
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Symbol:
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Tg(Col2a1-PTHLH)2Ecw
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Name:
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transgene insertion 2, Eleanor C Weir
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MGI ID: |
MGI:3717404 |
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Synonyms: |
col II-PTHrP |
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Transgene:
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Tg(Col2a1-PTHLH)2Ecw
Location:
unknown
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Skeletal phenotypes of wild type, Tg(Col2a1-PTHLH)2Ecw/0, Pthlhtm1Hmk/Pthlhtm1Hmk, and Pthlhtm1Hmk/Pthlhtm1Hmk Tg(Col2a1-PTHLH)2Ecw/0 mice
Show the 6 image(s) involving this allele.
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Transgene
origin |
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Strain of Origin:
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(C57BL/6 x SJL)F2
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Transgene
description |
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Transgene
Type: | |
Transgenic (random, expressed) |
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Mutation: | |
Insertion |
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Mutation details: A 568 bp fragment of human PTHLH cDNA under the control of the mouse Col2a1 promoter was used as the transgene. The Col2a1 promoter sequences were used to target expressioin of the transgene to proliferating and prehypertrophic chondrocytes. An artificial exon was engineered into the 5' end of the Col2a1 construct to prevent alternative splicing and the translation initiation codon was inactivated to ensure translation of the human PTHLH gene. 2.2 kb of human growth hormone untranslated sequences were added downstream of PTHLH to provide termination and polyadenylation signals as well as increase expression efficiency. This line exhibits lower expression levels than line 1. (J:76983)
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Phenotypes
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View phenotypes for all genotypes (concatenated display).
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available |
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| References |
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Original: |
J:50144
Philbrick WM et al.,
"Parathyroid hormone-related protein is required for tooth eruption."
Proc Natl Acad Sci U S A 1998 Sep 29;95(20):11846-51
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All: |
2 reference(s)
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Analysis Tools
